EMMPRIN与牙周炎的相关性研究

Expression of Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) in Periodontal Tissues

目的:研究EMMPRIN在牙周组织中的表达情况,分析其可能发挥的作用。方法:免疫组化、RT-PCR和western blot检测牙龈组织中EMMPRIN mRNA和蛋白表达情况。结果:临床健康和炎性牙龈组织中检测到EMMPRIN蛋白的表达,在炎性牙龈组织中的蛋白表达水平显著高于正常牙龈(P<0.05)。Pg LPS处理后的牙龈上皮细胞(HGEC)中EMMPRIN和MMPs mRNA的表达存在个体差异:来源于临床健康个体、牙龈炎和轻度牙周炎患者的HGEC在作用前后EMMPRIN和MMPs mRNA的表达无显著差异,而来源于重度牙周炎患者的HGEC在Pg LPS刺激后mRNA的水平显著上调(P<0.05)。结论:EMMPRIN可能参与牙周组织正常的生理改建;在炎症状态下EMMPRIN可能作为MMPs的诱导因子参与了对牙周组织的破坏,且与牙周炎的严重程度存在一定相关性。

Objective： To investigate the expression of EMMPRIN in periodontal tissue and determine its potential role during the progression of periodontitis. Methods： The EMMPRIN protein level was detected with immunohisto- chemistry（IHC） and western blot in gingival tissues from clinical healthy individuals and chromic periodontitis--affected patients. The human gingival epithelial cells （HGEC）were cultured from different periodontal status and dif- ferent objects. Then, reverse transcription-polymerase chain reaction （RT-PCR） was used to measure the mRNA level of EMMPRIN and MMPs in HGEC after treated with Porphyromonas gingivalis Lipopolysaccharides （Pg LPS）. Results： EMMPRIN protein was expressed in both healthy and inflamed periodontal tissues, and the level was significantly increased in inflamed ones. EMMPRIN mRNA, together with MMPs was expressed in untreated HGEC. Interestingly, their mRNA expression showed different reaction to Pg LPS. The mRNA of these four genes had no significant change after treatment in HGEC from healthy individuals or moderate periodontitis-affected patients, but it was upregulated significantly in HGEC from patients with severe periodontitis. Conclusion： EMM- PRIN was expressed in normal human gingival tissues and might play a role in the physiological remodeling of periodontal tissues.