硬骨鱼类干扰素调节因子4,8,9,10基因比较

Gene Comparative of Interferon Regulatory Factor (IRF) 4,8,9 and 10 in Teleost Fish

目的：为了阐明硬骨鱼类IRF4、IRF8、IRF0和IRF10在进化上的保守及分化。方法：从Ensembl和NCBI数据库下载基因的DNA和蛋白序列，绘制基因框架图，利用Mega5．0构建系统进化树，采用DNAStar计算蛋白相似率，利用Pfam软件分析保守结构域。结果：IRF8和IRFIO是相当保守的。各种硬骨鱼类中IRF8和IRF10基本一致。不同物种IRF8蛋白相似性最高（60％-87％），其次为IRF10（49～78％）。但IRF9和IRF4分化较大。硬骨鱼类有1—2个IRF9基因，1—3个IRF4基因。不同物种IRF4蛋白相似率为54—88％，IRF0更低（35—71％）。此外，在硬骨鱼类IRF9和IRF4蛋白上还发现有多种结构域。结论：硬骨鱼类IRF8和IRFIO非常保守，不同物种均含有8个外显子和7个内含子，蛋白相似性分别达到60％-87％和49。78％。IRF4和IRF0分化更大，不同物种基因数量从1变化到3，蛋白相似率低。

Objective：The conservation and diversification in evolution about IRF4, IRF8, IRF9 and IRF10 were elucidated in bony fishes. Method： Genomic and protein sequences were downloaded from the Ensembl and NCBI database, and then exon - intron structure, phylogenetic analyses, the identity of different protein and the conserved domains were studied. Result： IRF8 and IRF10 were conservative and quite comparable in all studied teleosts. IRF8 showed the highest identity between different teleosts （60% - 87% ）, followed by IRFIO （49%-78% ）. However, IRF4 and IRF9 diverged a lot. There had 1 -2 IRF9 genes and 1 -3 IRF4 genes. And the similarity of IRF4 between teleosts was 54 -88% while the identity between different IRF9 proteins was very low （35 -71% ）. Foremore, several do-mains were found in the IRF9 and IRF4 proteins. Conclusion：IRF8 and IRF10 were relative conservative in teleosts, with eight exons and seven introns in genomic structure and 60-87% and 49 -78% identity in proteins. IRF4 and IRF9 were diverged in gene number, exon-intron structure and low protein identity.