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依达拉奉对急性放射性损伤兔脑组织核转录因子-κB表达及丙二醛含量的影响 被引量：5

Effect of Edaravone on expression of nuclear transcription factor-κB and content of malondialdehyde in brain tissue of rabbits with acute radioactivity brain injury
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摘要 目的探讨依达拉奉对急性放射性损伤兔脑组织核转录因子-κB(NF-κB)表达及丙二醛(MDA)含量的影响。方法 54只新西兰兔随机分为空白对照组、实验对照组和实验用药组,每组18只兔子。用30 Gy钴60射线单次照射右侧半脑制成急性放射性脑损伤兔模型。实验用药组在造模前30 min腹腔注射依达拉奉1.5 mg/kg,造模后24 h与72 h分别重复注射1次。实验对照组兔予以等量生理盐水替代。空白对照组不予任何处理。采用HE染色观察各组兔子脑组织形态学变化,采用免疫组化染色法检测脑组织NF-κB表达,用比色法测定脑组织MDA含量。结果与空白对照组比较,实验对照组和实验用药组兔造模后各时间点脑组织NF-κB表达均明显增高,脑组织MDA含量明显增多(均P<0.05)。与实验对照组比较,实验用药组兔造模后72 h和7 d时,脑组织NF-κB表达明显降低,脑组织MDA含量明显减少(均P<0.05)。结论依达拉奉可以抑制急性放射性脑损伤后受损脑组织NF-κB的表达、降低其MDA含量,对急性放射性脑损伤具有一定的保护作用。 Objective To observe the effect of Edaravone on the expression of nuclear transcription factor （NF）-KB and content of malondialdehyde （MAD） in brain tissue of rabbits with acute radioactivity brain injury. Methods Fifty-four New Zealand rabbits were randomly divided into normal control group, experiment control group and experiment pharmacy group, with 18 rabbits in each group. Acute radioactivity brain injured rabbits models were caused by single fraction irradiation of 30 Gy Co60 ray on right half of brain. Experiment pharmacy group was given Edaravone 1.5 mg/kg by intraperitoneal injection at 30 min before experiment, and then injected again at 24 h and 72 h after experiment respectively. Experiment control group was given same dosage physiological saline. Normal control group was not given any treatment. The morphological change of cerebral tissue of each group was detected by HE staining. The expression of NF-KB was detected by immunohistochemieal staining, and the content of MAD was detected by colorimetric method. Results Compared with normal control group, the expression of NF-KB and the content of MAD were significantly increased at each time points of experiment control group and experiment pharmacy group （ all P 〈 0.05 ）. Compared with experiment control group, the expression of NF-~：B and the content of MAD were significantly decreased at 72 h and 7 d after experiment of experiment pharmacy group （all P 〈 0.05 ）. Conclusion Edaravone can inhibit the expression of NF-KB and reduce the content of MAD in brain tissue after acute radioactivity brain injury, which can take some protective effection on acute radioactivity brain injury.
作者 刘家斌 李广生 邓江华 王建
机构地区 江西赣州市人民医院神经内科
出处 《临床神经病学杂志》 CAS 北大核心 2013年第5期358-361,共4页 Journal of Clinical Neurology
关键词 急性放射性脑损伤 核转录因子-ΚB 丙二醛 依达拉奉 acute radioactivity brain injury nuclear transcription factor-KB malondialdehyde Edaravone
分类号 R818.7 [医药卫生—放射医学]
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参考文献7

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共引文献5

同被引文献71

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引证文献5

二级引证文献50

临床神经病学杂志
2013年 第5期

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