酸枣成熟胚离体培养研究

Culture of Mature Embryo of Sour Jujube(Zizyphus spinosa Hu.)in Vitro

为了建立酸枣成熟胚离体培养再生体系,以酸枣成熟胚为试验材料,研究启动培养基和增殖培养基对不同贮藏处理酸枣成熟胚萌芽率及增殖的影响。结果表明:以常温保存1a的成熟胚萌芽率较高(57.78%),最佳启动培养基为不添加任何植物激素的MS培养基,而最适增殖培养基为1/2MS+1.0mg/L 6-BA+0.3mg/L NAA+1.5g/L PVP。

The regeneration system of sour jujube （Zizyphus spinosa Hu. ） in vitro ,including the optimal initiation and proliferation medium, were investigated by using mature embryos as ex- plants to estimate the germination rate and proliferation rate. The results showed that the higher germination rate of mature embryos was obtained by cultivation of mature embryos kept a year at normal temperature on MS medium. The optimal medium for starting culture was hormone-free MS, The appropriate medium for proliferation of seedlings was 1/2MS medium supplemented with 1.0 mg/L 6-BA,0.3 mg/L NAA and 1.5 g/L PVP.