摘要
对由波兰小麦 DNA注射到普通小麦鄂恩 1号子房获得的 D5代稳定遗传变异 2个转化株系的种子醇溶蛋白 ,进行单向和双向聚丙烯酰胺凝胶电泳分析。结果表明由外源 DNA导入获得转化株系的变异 ,与其种子醇溶蛋白电泳图谱出现供体的某些组分和缺少受体的某些组分相印证。
Seed gliadins of the fifth generation (D 5) stably herditary variant types were obtained by injection of the total DNA of Triticum polonicum L.into the ovary of common wheat Triticum aestivum E en 1 and were analyzed by comparison of their one and two dimensional polyacrylamide gel electrophoretic patterns. The results showed that variation of transformation line was obtained by the introduction of exogenous DNA, accompanied with the appearance of some components of donor and the absence of some components of receptor on the electrophoretic patterns of seed gliadins proved each other.
出处
《武汉植物学研究》
CSCD
2000年第5期351-355,共5页
Journal of Wuhan Botanical Research
基金
国家"八 .五"科技攻关项目!(编号 :85-0 0 2 -0 2 -0 3)
湖北省"九 .五"农业重大科技项目!(编号 :982 P0 4 0 4 )资助课题
关键词
醇溶蛋白
外源DNA导入
转化株系
小麦
遗传变异
Gliadin, One and two dimensional electrophoresis, Introduction of exogenous DNA, Transformation line
