雷公藤多甙对银屑病患者NF-кB及细胞因子的影响

Effects of Glucosidorum Tripterygii Tororum on NF-κB and Cytokines of Patients with Psoriasis

目的:探讨雷公藤多甙对核因子的影响并为药物治疗银屑病提供理论依据。方法:密度梯度离心法获得外周血单一核细胞(PBMC),体外培养角质形成细胞(KC)。PBMC与KC混合培养,流式细胞仪检测核转录因子κB(NF-κB)表达,ELISA法测上清液白介素-8(IL-8)、细胞间黏附分子-1(ICAM-1)含量。结果:银屑病组NF-κB表达及ICAM-1、IL-8含量与正常对照组间差异有显著性(P<0.01);雷公藤可下调NF-κB表达及IL-8、ICAM-1含量(P<0.01)。结论:雷公藤可能通过影响NF-κB的表达而发挥对银屑病的治疗作用,抑制NF-κB的药物可能有助于银屑病的治疗。

Objective： To explore the effects ofglucosidorum tripterygii tororum on nuclear factor and provide theoretical evidence for treating psoriasis with drugs. Method： Peripheral blood mononuclear cells （PBMC） were obtained by density gradient centrifugation, and keratinocytes （KC） were cultured in vitro, PBMC and KC were mixed and cultured, the expressions of NF-KB were detected with flow cytometry and the contents of ICAM-1 and IL-8 in the liquid supernatant tested with ELISA method. Result： Psoriasis group showed significant difference compared with the normal control group in the expressions of NF-KB, the contents of ICAM-1 and IL-8（P〈0.01）; LeiGongTeng （tripterygium wilfordii） could lower the expressions of NF-κB and the contents of IL-8 and ICAM-1 （P〈0.01）. Conclusion： LeiGongTeng could improve the symptoms of psoriasis through influencing the expressions of NF-KB, drugs of inhibiting NF-KB might be helpful to the treatment of psoriasis.