摘要
摘要：建立了超高效液相测定天然β-胡萝卜索软胶囊中β-胡萝卜素的方法。采用ACQUITYUPLCBEHC18（2．1×50mm，1．7μm）色谱柱，以甲醇为流动相，流速为0．3mL/min，柱温为40℃，检测波长为450nm，β-胡萝卜素与其它组分分离良好。回收率在80％～90％之间。本方法简便快速，结果准确可靠。
A UPLC method for the determination of β-carotene in spirulina tablets was established. Using methanol as the mobile phase at flow rate 0.3 mL/min, the column temperature was 40 ℃, β- carotene was completely separated from other composition on a ACQUITY UPLC BEH C18 （2.1 ×50mm,1.7μm） ,then was detected at 450 nm. The average recovery for real samples ranged from 80% to 900/0. The method was simple,rapid,accurate and reliable.
出处
《江西科学》
2013年第5期596-598,共3页
Jiangxi Science
