摘要
目的：观察PPARy对大鼠脊髓损伤后神经干细胞分化为神经前体细胞影响，为脊髓损伤的防治提供理论基础。方法：使用清洁级sD大鼠108只，随机分成SCI组、RT组和GT治疗组。在不同时期内取材，行免疫荧光染色，观察PPAR？对大鼠脊髓损伤后神经干细胞分化为神经前体细胞的影响。结果：PPARy激动剂罗格列酮治疗组巢蛋白的表达较脊髓损伤组的巢蛋白表达在1～4w时间点内表达增加（P〈0．05），PPAR？拮抗剂GW9662组巢蛋白的表达较SCl组的巢蛋白表达在1～4w时间点内表达减少（P〈0．05），单因素方差分析表明三组之间具有统计学差异。结论：PPAR？能够促进神经干细胞分化为神经前体细胞，起到神经保护作用。
Objective： To investigate the influence of PPARy to NSCs differentiate into NSCs, so as to provide rationale basis for its prevention and cure. Methods： 108 rats were divided into spinal cord injury group, rosiglitazone therapy group, GW9662 therapy group, then immunofluorescence stain were performed to research the influence of PPARy to neural stem cells differentiate into neural precursor celles. Results： The expression ofnestin in rosiglitazone therapy group was more than spinal cord injury group, the expression ofnestin in GW9662 therapy group was less than spinal cord injury group （P〈0.05）, fator analysis of variance showed there is stastistical difference among three groups. Conclusion： PPARy has the effect of neuroprotetion by promoting the differenerentiation of NSCs into neural precursor cells, after activation ofrosiglitazone.
出处
《中医临床研究》
2013年第20期90-93,共4页
Clinical Journal Of Chinese Medicine
