格列卫诱导癌蛋白半乳凝集素-3通过溶酶体降解并促进胶质母细胞瘤U87细胞的凋亡

Gleevec induces lysosomal degradation of the oncoprotein Galectin-3 and promotes apoptosis in Glioblastoma U87 cell line

目的探讨格列卫诱导胶质母细胞瘤细胞株U87细胞凋亡及其促进癌蛋白半乳凝集素-3(Gal-3)降解的分子机制。方法利用细胞免疫染色法和提取细胞溶酶体的方法观察格列卫处理细胞后溶酶体中Gal-3蛋白量的变化;U87细胞分别用格列卫和/或STS及溶酶体抑制剂ConA处理后,用Western blot方法检测细胞中Gal-3蛋白量的改变;SubG1法检测U87细胞在格列卫及STS单药处理和联合处理时各组细胞凋亡率。结果格列卫处理U87细胞使Gal-3蛋白进入溶酶体并发生蛋白的降解;格列卫能够诱导U87细胞发生凋亡,与STS联合使用有协同诱导U87细胞凋亡的作用。结论格列卫能够诱导U87细胞株中Gal-3通过溶酶体降解,并使肿瘤细胞对凋亡诱导剂的敏感性增加。

Objective To investigate the effect of Gleevec promoting apoptosis of Glioblastoma U87 cell line and the molecular mechanisms of degradation of oncoprotein Galectin-3 （Gal-3）. Methods The quantity changes of Gal-3 protein in lysosomes was detected by immunostaining and extraction of lysosomes in Gleevec treated U87 cells ; the degradation of Gal-3 protein was detected by Western blot in Gleevec and/or stauroporine and/or ConA combine treated U87 cells ; the cell apoptosis ratio was accessed by SubG1 after U87 cells were treated with Gleevec and/or Stauroporine. Results Gal-3 protein gathered to lysosomes and degradated in lysosomes after U87 cells were treated with Gleevec;Gleevec could induce U87 apoptosis, and there was a synergistic effect between Gleevec and Stauroporine in inducing apoptosis. Conclusion Gleevec could induce lysosomal degradation of the oncoprotein Gal-3 and increase the sensitivity of U87 cells to apoptosis-inducing agent.