夏枯草酸性多糖及其衍生物的生物活性研究

Biological Activities of Acidic Polysaccharide and Its Derivatives from Prunella vulgaris Linn.

将分离纯化后的夏枯草酸性多糖分别用溶剂法脱硫、氯磺酸-吡啶法硫酸化修饰,然后用红外光谱初步分析夏枯草酸性多糖及其衍生物的结构特征,再对其抗氧化及免疫活性进行研究。结果表明,夏枯草酸性多糖(AP)含硫酸根6.58%,脱硫酸性多糖(APD)含硫酸根0.305%,硫酸化修饰后得到2种硫酸化产物低硫酸根含量硫酸酯化多糖(APL)和高硫酸根含量硫酸酯化多糖(APH),其硫酸根分别是30.95%和36.35%。几种多糖均具有一定的抗氧化活性,其抗氧化能力大小顺序依次为APH>APL>AP>APD。各多糖对ABTS·清除能力的半数抑制质量浓度(IC50)为:APD 4.96 g/L,AP 1.13 g/L,APL1.01 g/L和APH 0.57 g/L;对NO-2·清除能力的IC50分别为:APD 2.11 g/L,AP 1.59 g/L,APL 0.61 g/L和APH 0.34 g/L。几种多糖同时具有一定的免疫活性,且其免疫活性随多糖浓度和硫酸根含量的增加逐渐增强。

Acidic polysaccharide from Prunella vulgaris Linn. after isolation and purification was desulfated by solvent method and sulfated by chlorosulfonic acid-pyridine method, respectively. Then the structures of the acidic polysaccharide and its derivatives were preliminary investigated using IR spectrum. Their antioxidant activities and immunological activities were also determined. The result showed that the sulfate content of acidic polysaccharide （AP） from P. vulgaris was 6.58 %. The sulfate content of desulfate prtoduct （APD） was 0. 305 %. Two sulfated products （ sulfated polysaccharide with low sulfate content （APL） and sulfated polysaccharide with high sulfate content （APH）） were obtained from acidic polysaccharide after sulfated, and their sulfate content were 30.95 % and 36.35 %, respectively. Various polysaccharides showed significant antioxidant activities. The order of their antioxidant ability was APH 〉 APL 〉 AP 〉 APD. The ICs0 of various polysaccharides toward ABTS were APD 4.96 g/L,AP 1. 13 g/L, APL 1.01 g/L and APH 0. 57 g/L. The ICs0 of various polysaccharides toward NO2- were APD 2.11 g/L,AP 1. 59 g/L, APL 0. 61 g/L and APH 0. 34 g/L. Meanwhile, various polysaccharides represent significant immunological activities and their immunological activities were significantly enhanced with the increase of their concentrations and sulfate group content.