响应面法优化化香树果序提取物的提取条件研究

Extraction Conditions of Ellagic Tannin from Infructescence of Platycarya strobilacea Using Response Surface Methodology

在单因素试验的基础上,利用响应面分析方法优化化香树果序中单宁成分的提取工艺条件。利用中心组合设计研究提取时间、提取温度、液固比3个自变量对化香树果序提取物得率的影响。用Design-Expert 7.1软件进行结果分析。对于化香树果序提取物最佳提取工艺条件为:液固比17.5∶1(mL∶g),提取时间78 min,提取温度90℃,优化条件下化香树果序提取物得率为50.0%。

On the base of the single factor experiments, the conditions of the ellagie tannin extracted from infructescence of Platycarya strobilacea were optimized using response surface methodology （ RSM ）. A central composite design was used to investigate the effects of three independent variables, namely extraction time, extraction temperature and liquid to solid ratio on the yield of extract product. Data were analyzed using Design-Expert 7.1 software. The optimum extraction conditions were： the extraction time 78 min, extraction temperature 90 ℃, liquid to solid 17.5 ： 1 and extracting two times. ＇Under the optimum conditions, the extraction yield of tannin was 50.0%.