蛋白基因产物9.5在子宫内膜异位症患者在位及异位内膜中的表达情况

The Expression of Neuronal Protein Degradation Product 9.5 in Eutopic Endometrium and Ectopic Endometrium of Endometriosis

目的:探讨蛋白基因产物9.5(PGP 9.5)在子宫内膜异位症(EMs)患者的在位内膜以及异位内膜中的表达情况,并观察其表达量与EMs疼痛程度的关系。方法:应用免疫组化Envision二步法检测44例EMs患者(疼痛组28例,不痛组16例)的在位内膜、异位内膜及20例子宫肌瘤且不伴随疼痛的患者(对照组)子宫内膜中PGP 9.5的表达,探讨其与EMs患者痛经程度的相关性。结果:EMs患者在位内膜PGP 9.5的阳性表达率为72.9%,对照组在位内膜中PGP 9.5的阳性表达率为25.0%,差异有统计学意义(P<0.05)。EMs患者异位内膜中PGP 9.5的阳性表达率为81.8%;其中疼痛组患者的阳性表达率为92.9%,不痛组的阳性表达率为62.5%,两者相比差异有统计学意义(P<0.05)。EMs组在位内膜和异位内膜神经纤维密度均高于对照组(均P<0.01)。PGP 9.5的表达与EMs的分期以及发生部位无关(均P>0.05);EMs患者疼痛与病灶发生部位无关(P>0.05),且与神经纤维密度无关(P>0.05)。结论:PGP 9.5在EMs患者在位以及异位内膜中阳性率显著增高,同时其表达量增高,与患者痛经程度有一定相关性。

Objective：To investigate the expression of protein gene product 9.5 （PGP 9.5） in situ function layer and ectopic endometrium of endometriosis, and observe the relationship of the expression of PGP 9.5 and the pelvic pain. Methods： 44 endometriosis patients enrolled in this study （pain group 28 cases ,without pain group 16 cases） ,to detect the expression of PGP 9.5 in situ function layer and ectopic endometrium of patients were determined by immunohistochemical methods of Envision TM ,20 patients with uterine fibroid without pelvic pain were included as the control group. Investigate the relationship of PGP 9.5 in endometriosis and the PGP 9.5 and the pelvic pain. Results：The positive rate of PGP 9.5 expression in endometriosis group situ function layer was 72.9%, and the positive rate of PGP 9.5 expression in control group situ function layer was 25.0% ,which has significant difference （P〈0.05）. The positive rate of PGP 9.5 expression in EMs group ectopic endometrium was 81.8% ,and the positive rate of PGP 9.5 expression in endometriosis pain group ectopic endometrium was 92.9% ,compared with the without pain group 62.5%. Which has significant different （P〈0.05）. EMs in ectopic group and ectopic endometrial nerve fiber density were higher than control group （all P〈0.01 ）. The expression of PGP 9.5 has nothing to do with the staging and location of the EMs （all P〉0.05）. EMs patients＇ pain has nothing to do with the lesion location （P〉 0.05）,and has nothing to do with the density of nerve fibers （P〉0.05）. Conclnsions：The expression of PGP 9.5 had a significant increase in the endometriosis situ function layer and ectopic endometrium. At the same time, its increased expression had a close relationship with the pelvic pain.