血管紧张素-(1-7)对胰岛素分泌细胞株NIT-1增殖的影响

Effect of Angiotensin-(1-7) on Proliferation of Insulin-secreting Cell Line NIT-1

[目的]探讨血管紧张素[Ang-（1-7）]对小鼠胰岛素分泌细胞株NIT-1细胞增殖的影响.[方法]NIT-1细胞按以下分组分别处理24 h,采用CCK-8比色法检测细胞增殖.（1）11.1、25.0、30.0、35.0和40.0 mmol/L葡萄糖液,35.0 mmol/L甘露醇分别处理24 h;（2）0、10-7、10-6、10-5和10-4 mol/L Ang-（1-7）分别处理24 h;（3）高糖（HG,35.0 mmol/L）、HG＋Ang-（1-7）（10-5 mol/L）、HG＋Ang-（1-7）＋Mas受体拮抗剂（A-779,10-5 mol/L）和HG＋A-779组.[结果]（1）较11.1 mmol/L组,葡萄糖浓度为35.0和40.0 mmol/L时,NIT-1细胞的增殖明显减低（1.02±0.07 vs 1.21±0.10;0.90±0.05 vs 1.21±0.10,P＜0.05）.（2）在11.1 mmol/L葡萄糖培养条件下,10-7～10-4mol/L之间的Ang-（1-7）不影响NIT-1细胞的增殖.（3）与HG组相比,HG＋Ang-（1-7）组细胞的增殖率明显增加（1.44±0.24 vs 1.14±0.07,P＜0.05）,加入A-779共同孵育可逆转Ang-（1-7）的促增殖效应（1.20±0.02 vs 1.44±0.24,P＜0.05）.[结论]Ang-（1-7）通过Mas受体拮抗高糖对NIT-1细胞增殖的抑制作用.

[ Objective ] To investigate whether Angiotensin-（ 1-7 ） can affect the proliferation of mouse insulin-secreting cell line NIT-1. [ Methods ] NIT-1 cells were divided into different groups as follows and cell counting Kit-8 method （CCK-8） was performed to detect the proliferation of NIT-1 at different groups separately： （1） Glucose at concentration of 11.1, 25.0, 30.0, 35.0, and 40.0 mmol/L, mannitol at concentration of 35 mmol/L for 24 h; （2） Ang-（ 1-7 ） at concentrations of 0, 10-7, 104, 10-5, and 10-4 mol/L for 24 h; （3） High glucose（HG）, HG＋Ang-（1-7）, HG＋Ang-（1-7） ＋Mas receptor inhibitor（A-779）, HG＋A-779. [Results] （1） Compared with the 11.1 mmol/L group, the cell proliferation were inhibited in the 35.0 mmol/L and 40.0 mmol/L groups （ 1.02 ± 0.07 vs 1.21± 0.10; 0.90 ± 0.05 vs 1.21 ± 0.10, P 〈 0.05）. （2） No significant difference of the proliferation in NIT-1 cells was found between Ang-（1-7） treatment groups （10.7 ± 10.4 mol/L）. （3） Compared to the HG group, the proliferation of the HG＋Ang- （1-7） group was higher （1.14 ±0.07 vs 1.44 ± 0.24, P 〈 0.05）; Compared to the HG＋Ang-（1-7） group, the proliferation of the HG＋Ang-（ 1-7）＋A-779 group was decreased （1.44±0.24 vs 1.20 ± 0.02,P 〈 0.05）. [Conclusion] By binding to Mas receptor, Ang-（ 1-7 ） could reverse the inhibitory effect of high glucose on proliferation of NIT-1 cells.