环介导等温扩增技术及其在食源性吸虫感染检测中的应用

Principle of loop-mediated isothermal amplification technique and its application in detection of food-borne trematode infection

随着人们生活水平的提高,土源性寄生虫病的感染率和发病率日趋下降,与之相反,食源性寄生虫病更加引人关注,而食源性吸虫病是其中的重要组成。据WHO统计,目前已知能感染人体的食源性吸虫有100多种,感染者超过4000万人,还有10%的人口面临感染食源性吸虫的风险。目前对于腔道内寄生的食源性吸虫通常采用病原学诊断方法,但对于深部组织内寄生的食源性吸虫则缺乏有效的诊断方法。环介导等温扩增(LAMP)技术是新近发展起来的一种方法,它具有简便快速、灵敏特异、经济实用、且易于普及等优点,有替代传统PCR之势。但国内对LAMP法的了解和应用不多,基于其在食源性吸虫感染方面的诊断价值,本文予以介绍,以供借鉴。

Along with people＇s living standard improved, the rates of infection and morbidity of soil-transmitted parasito- sis are in declining gradually. In contrast, food-borne parasitosis isof great concern. Thereinto, food-borne trematodiasis is a very important component part. According to WHO statistics, currently food-borne trematodes have more than 100 species to infect human. The number of people infected exceeds 40 million, and 10% of the population is at risk of food-borne trematodes infection. At present, food-borne trematodiases located in intracavity are usually detected by etiology diagnosis methods, but deep tissue entozoic food-borne trematodes are short of effective diagnostic methods. As one of the new methods, loop-mediated isothermal amplification technique has obvious advantages of being easy, rapid, sensitive, specific, economic, practical, popu- lar etc. , and is on the trend of replacing traditional PCR. In the domestic academic circles, people actually know less about its understanding and application. Based on its significantly diagnostic value in detection of food-borne trematodes infection, it is worthy to introduce the technique for reference.