均匀设计法优化金线吊葫芦黄酮类化合物乙醇提取工艺

Processing optimization of ethanol extracting of flavonoid from Wahlenbergia marginata ( Thunb. ) A. DC. by uniform experiment

以提高金线吊葫芦提取液中黄酮类化合物质量浓度为考察指标,采用单因素试验方案探讨乙醇体积分数、提取温度及提取时间等对提取液中黄酮类化合物浓度的影响,应用均匀试验设计方案对影响提取液中黄酮类化合物浓度的主要因素水平进行优化.结果表明:优化的因素水平组合为乙醇体积分数55%,提取温度74℃,提取时间50 min,黄酮类化合物质量浓度为0.106 g·L-1.

To improve the concentration of flavonoid compounds in the Wahlenbergla marg/nata （Thunb.） A. DC. extraction liquid, influences of ethanol concentration, extraction time and extraction temperature on the absorbance value were analyzed by using the single factor experiment. Orthogonal experimental design was applied to optimize the main factors that affected the concentration of flavonoids in the extract. The results indicated that the optimized factors were 55% of ethanol concentration, 74℃ of extraction temperature and 50 rain of extraction time.