电离辐射致大鼠肠上皮细胞磷脂变化的研究

Alternations of phospholipids in the rat intestinal epithelial cells after ionizing radiation

目的观察电离辐射所致肠上皮细胞磷脂的变化，探讨放射性肠损伤的发生机制。方法将大鼠小肠上皮细胞（IEC-6）分为3组：正常对照组、8Gyx射线照射组和12Gyx射线照射组。分别在照射后6和24h，提取IEC-6细胞磷脂，利用液相色谱-质谱联用技术（HPLC．MS）测定辐射所致细胞磷脂的变化。结果辐射后6h，8Gy照射组细胞磷脂未发生显著变化；12Gy照射组细胞磷脂变化明显，其中磷脂酰甘油（PC）、磷脂酰肌醇（PI）和溶血磷脂酰胆碱（LysoPC）均显著性上调（F=5．37、9．60、9．88，P〈0．05）。而照射后24h，两个辐射剂量组中多种磷脂酰乙醇胺（PE）和PC分子显著下调（F=5．15～99．77，P〈0．05），12Gy照射组中多种神经鞘磷脂（SM）显著上调（F＝4．35—7．92，P〈0．05）。结论电离辐射可导致大鼠肠上皮细胞（IEC-6）磷脂代谢紊乱，其紊乱程度与辐射剂量相关。

Objective To investigate radiation-induced alternations of phospholipids in epithelial cells, and to provide experimental evidence for understanding the mechanism of radiation-induced intestinal injury. Methods The intestinal epithelial cells （IEC-6） in rats were divided into three groups： normal control group, 8 Gy X-ray irradiation group and 12 Gy X-ray irradiation group. Phospholipids were extracted at 6 h or 24 h after radiation and then measured by high-performance liquid chromatography and mass spectrometry （HPLC-MS）. Results At 6 h after radiation, the phospholipids in 8 Gy irradiation group didn＇t vary significantly, while those in 12 Gy irradiation group changed. The PG, PI and Lyso PC were significantly up-regulated （ F = 5.37, 9.60, 9.88, P 〈 0. 05）. However, at 24 h after radiation, many PE and PG species in both irradiation groups declined （ F = 5.15 - 99.77, P 〈 0. 05 ） and SM species increased in 12 Gy irradiation group （ F = 4. 35 - 7.92, P 〈 0.05 ）. Conclusions The ionizing radiation could disorder phospholipid metabolism in IEC-6 cells with a dose-dependent manner.