摘要
目的探讨RNA干扰Toll样受体4(Toll—likereceptor4,TLR4)信号通路对枯否细胞(Kupffercells,KCs)吞噬功能的影响。方法以RAW264.7小鼠单核巨噬细胞白血病细胞为研究对象,经QPCR验证得到最优干扰RNA为Tlr4-mus-1567后,以正常细胞为对照组,NegativeControlRNA干扰为NC对照组,Tll4-mus-1567RNA干扰为实验组,倒置显微镜观察各组细胞吞噬酵母菌情况,并测定各组的吞噬细胞百分数和吞噬指数,组间进行比较。结果实验组平均吞噬细胞百分数较正常对照组明显降低(17.67%±3.51%和32.00%±3.00%,P〈0.01),实验组平均吞噬指数较正常对照组明显降低(46.33%±7.51%和82.00%±6.08%,P〈0.01)。结论RNA干扰TLR4信号通路能降低KCs的吞噬功能。
Objective To investigate the effect of RNA interfering TLR4 signal pathway on phagocytosis of Kupffer cells. Methods RAW2647 mice mononuclear macrophage leukemia cells were observed. The tested group was interfered by Tlr4-mus-1567 RNA which had the best result confirmed by QPCR, cells interfered by Negative Control RNA as NC group, and normal cell as control. We perform the phagocytosis test on each group. Results The tested group has lower phagocytes percentage than control (17.67% ±3.51% vs 32.00%±3.00% , P〈0.01), and lower phagocytic index(46.33% ±7.51% vs 82.00% ± 6. 08% , P 〈0. 01 ). Conclusions Decreased phagocytic activity was observed on Kupffer cells by RNA interference.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2013年第5期322-324,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金项目,浙江省自然科学基金项目