Ⅲ型前胶原氨基端肽的化学发光定量检测

Quantitative detection of serum procollagen Ⅲ N-terminal peptide chemiluminescence enzyme immunoassay

目的建立人血清中Ⅲ型前胶原氨基端肽（PⅢNP）的定量微孔板化学发光酶免疫检测方法。方法辣根过氧化物酶标记PⅢNP单抗，选用鲁米诺化学发光体系检测，优化各类反应液的工作浓度和各种反应条件，建立双抗体夹心的检测方法；同时评价所建立方法的灵敏度、特异性、线性范围、稳定性等性能指标；并应用临床血清与进口试剂进行比对实验。结果所建立方法的线性范围为0．8—85ng／ml，灵敏度0．5rig／ml，批内、批间变异均〈10％。检测PⅢNP的临床高、中、低值血清回收率分别为96．2％、91．2％和101．1％；在4℃和37℃条件下分别进行了3d、5d．7d的稳定性考察，线性相关系数均〉0．99，标准偏差〈6％；比对实验分析显示与进口试剂相关性具有统计学意义。结论成功建立了定量PmNP化学发光酶免疫分析方法，并且有较好的准确性、灵敏度、重复性，与进口试剂检测结果等效。

Objective To establish chemiluminescence enzyme immunoassay （CLEIA） for quantitative detection of procollagen Ⅲ N-terminal peptide （ P Ⅲ NP） in serum. Methods A sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of P RI NP as the catalytic enzyme and the luminol as the luminescence reagent. Several reactions liquid＇s concentration and reaction conditions were optimized. The method was evaluated in all aspects such as linear range, sensitivity, specificity, stability and so on. The CLEIA was compared with imported ELISA kits, by detecting clinical serum. Results The linear range was 0. 8-85 ng/ml. The detection limit was 0. 5 ng/ml. Inter-assay and intra-assay RSD were both less than 10%. The recoveries of three different spiked concentration samples were 96. 2% , 91.2% and 101.1%. After stored at 4℃ and 37℃ for 3, 5, 7 days, the analysis showed correlation coefficient higher than 0. 99 and RSD lower than 6%. The detected results of clinical sera with CLEIA closely corresponded to those with imported ELISA. Conclusion Established CLEIA for quantity determination of serum PHI NP has high accuracy, sensitivity and repeatability.