生物可降解Mg-2Zn-0.2Mn合金的体外生物相容性

In vitro Biocompatibility of Biodegradable Mg-2Zn-0.2Mn Alloy

采用真空铸造技术制备了Mg-2Zn-0.2Mn三元镁合金,并对该合金体外生物生物相容性能进行了研究。采用血小板粘附、溶血率评价镁合金的血液相容性,采用细胞增殖实验(CCK-8)、细胞凋亡实验(AO/PI)评价镁合金的细胞相容性。结果表明:血小板粘附实验表明,与常用的AZ31B镁合金和316L不锈钢样品相比较,Mg-2Zn0.2Mn镁合金表面粘附的血小板数量少,且激活和团聚较轻微;溶血实验的结果表明Mg-2Zn0.2Mn的溶血率为4.7%,远小于AZ31B的80.9%溶血率。血小板黏附和溶血实验说明Mg-2Zn0.2Mn镁合金具有优异的血液相容性。细胞增殖和凋亡实验结果也表明,较AZ31B样品,Mg-2Zn0.2Mn样品表面显示了更好的内皮细胞粘附和增殖能力,说明Mg-2Zn0.2Mn镁合金具有很好的血液相容性和内皮细胞相容性,有望作为今后可降解血管支架的候选材料。

High purity magnesium alloy （Mg-2Zn-0.2Mn） with 2 wt% zinc （Zn） and 0.2 wt% manganese （Mn） was prepared by a vacuum casting technology in order to investigate its in vitro biocompatibility. The hemocompatibility was evaluated by platelet adhesion and hemolysis ratio testing, and the cytocompatibility was studied by cell proliferation test （CCK-8） and apoptosis experiment （AO/PI）. The results show that significantly fewer platelets were adherent and activated on the Mg-2Zn-0.2Mn surface than on the controls of AZa1B magnesium alloy and 316L stainless steel. And the hemolysis ratio of Mg-2Zn-0.2Mn was 4.7%, which was much less than that of AZ31B （80. 9G）. The behavior of endothelial cells adhesion and proliferation on Mg-2Zn-0.2Mn were superior to AZ31B. It is concluded that Mg-2Zn-0.2Mn is a promising candidate for future biodegradable stent because of its excellent hemocompatibility and cytocompatibility.