视网膜干细胞培养上清液诱导骨髓间充质干细胞的分化

Retinal stem cell culture supernatant induces differentiation of bone marrow mesenchymal stem cells

背景:骨髓间充质干细胞向神经细胞诱导分化可为神经系统受损伤后的修复和再生带来了新的希望。目的:探讨骨髓间充质干细胞在视网膜干细胞培养上清液诱导条件下向神经元细胞分化。方法:采用全骨髓培养方法,用视网膜干细胞培养上清液诱导骨髓间充质干细胞,通过免疫荧光染色鉴定其分化的结果。结果与结论:诱导72 h,骨髓间充质干细胞表达神经干细胞的特异性抗体巢蛋白和神经元中的标志性微管相关蛋白微管相关蛋白2。视网膜干细胞培养上清液能够促进骨髓间充质干细胞向神经元样细胞分化,提示视网膜干细胞可能分泌神经生长因子。

BACKGROUND： Differentiation of bone marrow mesenchymal stern cells into nerve cells has brought a new hope for the repair and regeneration after nervous system injuries. OBJECTIVE： To study the neuron-like differentiation of bone marrow mesenchymal stem cells induced by the retinal stem cell culture supernatant. METHODS： The whole bone marrow culture was applied. The differentiation results of bone marrow mesenchymal stem cells induced by retinal stem cell culture supernatants were identified by immunofluorescence method. RESULTS AND CONCLUSION： After 72 hours of induction, bone marrow mesenchymal stem cells express nestin and microtubule-associated protein 2. Retinal stern cell culture supernatant can promote bone marrow mesenchymal stem cells differentiating into neuron-like ceils, suggesting that retinal stem ceils may secrete nerve growth factor.