碱性成纤维细胞生长因子缓释体系诱导脂肪移植体早期血运重建

Early revascularization of fat grafts conducted by basic fibroblast growth factor sustained-release system

背景:脂肪移植吸收及其不可预测性直接影响该技术的临床应用,利用各种细胞因子来促进脂肪移植体早期血运重建,从而减少移植体吸收的技术在目前也有待提高。效果不稳定在于这些细胞因子在术后随血液和渗出液的流失而不能在较长时间内有效作用于血管内皮细胞。目的:探讨碱性成纤维细胞生长因子与葡聚糖颗粒形成缓释体对脂肪移植体早期血运重建的影响。方法:制备2 mg/L的碱性成纤维细胞生长因子溶液及其同浓度的碱性成纤维细胞生长因子-葡聚糖缓释颗粒。SD大鼠12只取腹股沟脂肪行自体脂肪移植,左侧背部皮下植入脂肪珠加入重组型碱性成纤维细胞生长因子溶液(对照组),右侧背部皮下植入脂肪珠加碱性成纤维细胞生长因子-葡聚糖缓释颗粒(缓释组),在7,14 d随机方法处死动物各1只,用墨汁灌注微血管显像法观察移植体内部和包膜血管早期生成密度。移植后90 d,将剩余大鼠处死,取移植体测量体积。结果与结论:在植入后7,14 d,包膜和移植体内血管计数缓释组均高于对照组(P<0.01)。90 d后缓释组移植体的体积大于对照组(P<0.05)。碱性成纤维细胞生长因子缓释体可以更好地促进脂肪移植体早期血运重建。实验结果说明,碱性成纤维细胞生长因子缓释体有利于减少术后脂肪移植体的吸收。

BACKGROUND： The resorption and unprediction of fat transplantation directly influence the clinical application. The technology of reducing graft resorption by using various cytokines to promote early revascularization of fat transplantation needs to be improved. But the effect is not stable, as the cytokines losing with the bleeding and exudation thus cannot long-term affect the vascular endothelial cells. OBJECTIVE： TO investigate the effect of alkaline recombinant basic fibroblast growth factor-dextran formed sustained-release system on the early revascularization of fat grafts. METHODS： The recombinant basic fibroblast growth factor solution 2 mg/L and basic fibroblast growth factor-dextran sustained-release granules were prepared. The fat tissues were selected from the inguinal region of 12 Sprague-Dawley rats for fat transplantation. The left backs of the rats were treated with subcutaneously injection of fat pearl plus recombinant basic fibroblast growth factor solution （control group）, and the right backs of rats were treated with subcutaneously injection of fat pearl plus basic fibroblast growth factor-dextransustained-release granules （sustained-release system group）. At 7 and 14 days after treatment, one rat in each group was sacrificed randomly, then ink perfusion microvascular imaging was used to observe the inside of the graft and the density of early formed coated vessels. At 90 days after transplantation, the rest rats were sacrificed, and the grafts were obtained to measure the volume. RESULTS AND CONCLUSION： At 7 and 14 days after transplantation, the number of vessels in the capsule and grafts of the sustained-release system group was higher than that in the control group （P 〈 0.01 ）. At 90 days after transplantation, the volume of grafts in the sustained-release system groups was bigger than that in the control group （P 〈 0.05）. The recombinant basic fibroblast growth factor-dextran delivery system could better promote the early revascularization of fat grafts. The experimental results suggest that recombinant basic fibroblast growth factor-dextran sustained-release system can help to reduce the absorption of fat grafts after surgery.