3-溴丙酮酸对兔VX2肝肿瘤转移及兔生存时间的影响

Effects of intra-arterial infusion of 3-bromopyruvate on metastases and survival benefit of hepatic VX2 tumor in rabbits

目的探讨3-溴丙酮酸（3-BrPA）经肝动脉灌注对兔VX2肝肿瘤转移及荷瘤兔生存时间的影响。方法18只新西兰大白兔肝左叶种植VX2肿瘤，随机分成3组，每组6只。PBS灌注组：在肿瘤种植14d后行肝动脉PBS溶液灌注。3-BrPA7和14d灌注组：在肿瘤种植7／14d后行肝动脉3-BrPA溶液灌注。在肿瘤种植28d后每组处死3只兔，解剖探查有无肝内转移、肾转移、肺转移及腹腔转移。每组剩余的3只兔观察其生存时间并进行比较。结果肿瘤种植28d后，PBS灌注组均发现肝内及腹腔转移（3／3），肾转移2只（2／3），肺转移2只（2／3）。3-BrPA7d灌注组实验兔肝内和肺转移各有1只（1／3），未发现有腹腔和肾转移（0／3）。3-BrPAl4d灌注组实验兔有2只发现肝内转移（2／3），肺和腹腔转移各有1只（1／3），未发现肾转移（0／3）。生存时间比较显示3-BrPA14d灌注组实验兔生存时间[（27±5）d]显著长于PBS溶液灌注组[（17±3）d]（P=0．041）。而3-BrPA7d灌注组实验兔生存时间[（42±6）d]显著长于3-BrPA14d灌注组实验兔[（27±5）d]（P=0．007）。结论经肝动脉灌3-BrPA能够有效减少兔VX2肝肿瘤的转移，并可延长移植VX2肝肿瘤兔的生存时间，且灌注时间越早，治疗效果越好。

Objective To evaluate the metastasis and survival of an intra-arterial infusion of 3- bromopyruvate （3-BrPA） on hepatic ~X2 tumor in rabbits. Methods VX2 tumor was implanted in left lateral lobe of liver of 18 white New Zealand rabbits. The animals were randomized into 3 groups （ n = 6each） and underwent an intra-arterial infusion of phosphate-buffered saline or 3-BrPA via hepatic artery at 14 days post-implantation. At 28 days post-implantation, 3 rabbits in each group were sacrificed. The abdomen of these rabbits was opened and inspected for metastases. Then the survival of the remaining rabbits was observed. Results At 28 days post-implantation, in PBS group, there were intrahepatic metastasis and abdominal cavity dissemination （ n = 3 ） , renal metastases （ n = 2 ） and lung metastases （ n = 2 ） ; in early 3- BrPA infusion group, intrahepatie metastasis （ n = 2 ）, abdominal cavity dissemination （ n = 1 ） and lung metastases （n = 1 ） ; in late 3-BrPA infusion group, intrahepatic metastasis （ n = 1 ） and lung metastases （n = 1 ）. The survival of the remaining animals was observed. Rabbits in early 3-BrPA infusion group survived significantly longer than those in PBS group [ （ 27 ± 5 ） vs （ 17 ± 3 ） days, P = 0. 041 ] ; rabbits in late 3-BrPA infusion group [ （42 ± 6） days] survived significantly longer than those in early 3-BrPA infusion group （P = 0. 007）. Conclusion An intra-arterial infusion of 3-BrPA could reduce metastasis and prolong survival in rabbits with hepatic VX2 tumor. The earlier the infusion, the better the outcome.