银杏叶提取物对激光诱导的大鼠脉络膜新生血管的疗效

Effects of ginkgo biloba extract on laser-induced choroidal neovascularization in rats

目的:观察银杏叶提取物对激光诱导的大鼠脉络膜新生血管(choroidal neovascularization,CNV)的疗效。方法:选取60只大鼠,随机分为4组,分别为正常组、模型组、实验组、生理盐水组,每组15只大鼠。除正常组外其余各组均行氪激光光凝制作CNV模型,模型组不做任何处理,实验组均在光凝后每日行腹腔注射银杏叶提取物(Extract of Ginkgo Biloba,EGb761),生理盐水组在光凝后每日腹腔注射生理盐水。于光凝后7,14,21d后对各组大鼠行眼底造影检查,观察CNV的渗漏情况,然后立即处死各组动物,行脉络膜视网膜切片,HE染色后观察各组视网膜及脉络膜的结构和CNV的情况。结果:荧光造影结果显示正常大鼠脉络膜无渗漏,7d时各组大鼠脉络膜渗漏均较轻微,14,21d时模型组和生理盐水组大鼠脉络膜渗漏明显,实验组大鼠脉络膜荧光素渗漏点数明显要少于对应的模型组和生理盐水组差异具有统计学意义(P<0.05)。实验组大鼠的视网膜、脉络膜结构保存比模型组更好。结论:腹腔注射EGb761可以明显减少大鼠CNV的形成,用药时间越长,疗效越好。

AIM To investigate the effects of ginkgo biloba extract （EGb 761 ） on laser-induced choroidal neovascularization （CNV） in rats. ~ METHODS： Totally 60 BN rats were randomly divided into 4 groups= normal control group, model group, experimental group, physiological saline group with 15 in each group. All CNV models were made by krypton laser. Rats in experimental group were intraperitoneally injected with 0.35% EGb761 （100mg/kg） every day after laser exposure until they were sacrificed. Rats in physiological saline group were intraperitoneally injected physiological saline every day after laser exposure until they were sacrificed. Fundus fluorescein angiography （FFA） was performed on every rat on the 7th day, 14th day and the 21 st day after laser exposure, then the rats were sacrificed immediately. The eyes were enucleated and processed for histopathologic examination. ~ RESULTS= There was no choroidal fluorescein leakage staining in normal rats. There were obviously less choroidal fluorescein leakage points in experimentalgroups than that in the corresponding model groups （H〈 0. 05）, the difference had statistical significance. The structures of the retina and choroid were preserved better in experimental group than that in the model group. ~ CONCLUSION. EGb761 len inhibit the formation of laser-induced CNV in rats. The longer the time, the better curative effect.