CD4^+CD25^+调节性T细胞在Graves眼病患者外周血中的表达

Detection of CD4^+CD25^+ regulatory T cell in patients with Graves' ophthalmopathy

目的:通过检测CD4+CD25+调节性T细胞在Graves眼病患者外周血中的表达,以观察该病患者外周血中CD4+CD25+调节性T细胞的变化规律。方法:临床实验研究。采用流式细胞术检测对Graves病不伴有眼征患者组(53例),Graves眼病患者组(51例),正常对照组(51例)的外周静脉血CD4+CD25+Treg细胞比例进行检测。结果:与正常对照组比较,Graves病不伴有眼征患者组Treg水平下降(P<0.01);与正常对照组比较,Graves眼病患者组外周血Treg水平显著下降(P<0.01);Graves眼病患者组外周血Treg数量明显低于Graves病不伴有眼征患者组(P<0.01)。结论:Graves眼病患者外周血中CD4+CD25+Treg在淋巴细胞中所占的比例降低,存在自身免疫调节紊乱。可能是其机体细胞免疫抑制受损的重要机制,为对该疾病进行免疫学治疗提供了新线索。

AIM： To observe the change law of the ratio of CD4＊ CD25＋ regulatory T cell in the peripheral blood of the patients with Graves＇ ophthalmopathy by detecting the ratio of this cell. ~ METHODS： This experimental study selected 53 GO patients without exophthalmos, 51 GO patients with exophthalmos, and 51 healthy people were collected. The ratio of CD4＋ CD25＋ T cell in peripheral blood was determined by flow cytometry. ~ RESULTS： The ratio of CD4＊CD25＋ T cell in GO patients without exophthalmos was lower than that in healthy group （P〈0.01~）. The ratio of CD4＋CD25~ T cell in GO patients with exophthalmos was far lower than that in healthy group （ P〈0.01 ）. Compared with the group of GO patients without exophthalmos, the ratio of CD4~ CD25＋ T cell of GO patients with exophthalmos was also much lower （ P〈0.01 ）. ~ CONCLUSION ： The ratio of CD4＋ CD25＋ T cell in the GO patients decreases and there is autoimmune disorder in patients of this disease. Perhaps this is an important mechanism causing immune suppression damage, which provides a new clue for immunological treatment.