大鼠蛛网膜下腔出血后脑组织脂连素受体表达和血清脂连素质量浓度的变化

Changes of expressions of AdipoR in the central nervous system and adiponectin serum concentrations after subarachnoid hemorrhage in rats

目的探讨大鼠蛛网膜下腔出血(SAH)后脑皮层和海马脂连素受体(AdipoR)表达的改变和血清中脂连素质量浓度的变化。方法将54只SD大鼠随机分为正常组(n=12)、假手术组(n=12)、SAH模型组(n=30)。采用颈内动脉穿刺法制作大鼠SAH模型。通过ELISA检测血清中脂连素的质量浓度;采用Western blot检测脂连素受体表达的变化。结果与正常组和假手术组相比,SAH后1、2、3d时血清脂连素浓度明显降低(P<0.05)。与正常组和假手术组相比,SAH模型组大鼠皮层和海马的脂连素受体1(AdipoR1)的表达明显升高(P<0.05),而脂连素受体2(AdipoR2)表达无明显变化。结论脂连素参与了SAH后早期脑损伤的病理生理过程,血清脂连素质量浓度的降低以及AdipoR1表达的增多,可能都是导致SAH后早期脑损伤的重要病理机制。

Objective To investigate changes of AdipoR in the central nervous system and concentration of serum adiponectin after subarachnoid hemorrhage （SAH） in rats, Methods Fifty-four male Sprague-Dawley rats were randomly divided into three groups： normal group （n- 12）, sham-operation group（ n = 12）, and SAH model group （n =30）. An endovascular perforation model was used to induce SAH in the rats. The expression of AdipoR in the central nervous system was detected by Western blot. The concentration of serum adiponectin was detected by ELISA. Results Compared with that in normal group and sham-operation group, the concentration of serum adiponectin was decreased significantly at day 1, 2 and 3 after SAH （P〈O. 05）. Compared with that in normal group and sham-operation group, the expression of AdipoR1 in the cerebral cortex and the hippocampus was up- regulated sharply after SAH （P〈0.05）, but the expression of AdipoR2 did not change significantly （P〉 O. 05）. Conclusion Adiponectin may be involved in the pathophysiological process of early brain injury after SAH. The decreased concentration of serum adiponectin and the up-regulation of AdipoR1 expression are both important pathological mechanisms leading to early brain injury after SAH.