Sox-2及Wnt信号通路蛋白在宫颈癌中的表达及相互作用

Relationship between Sox-2 and Wnt signaling pathway in cervical cancer

目的探究干细胞相关基因Sox-2和Wnt信号通路蛋白在宫颈鳞癌中的表达及二者的相关性。方法采用免疫细胞化学方法检测Sox-2、β-catenin及C-myc在宫颈癌细胞系HeLa、SiHa、C33A中的表达;采用免疫组织化学法检测其在30例正常宫颈组织及43例宫颈鳞癌组织中的表达。结果 Sox-2在SiHa、C33A的细胞核中表达,在HeLa中未见表达;β-catenin及C-myc在HeLa、SiHa及C33A中均有表达。在宫颈鳞癌组织中Sox-2、β-catenin和C-myc的表达明显高于正常宫颈组织(P<0.05);Sox-2表达与宫颈鳞癌病理分级相关(P=0.049),与临床分期、淋巴结转移、年龄无相关性(P>0.05),β-catenin和C-myc的表达与病理分级、临床分期、淋巴结转移及年龄均无关(P>0.05)。在宫颈鳞癌中,Sox-2与β-catenin的表达呈正相关(P<0.001,r=0.567),与C-myc的表达也呈正相关(P=0.030,r=0.331)。结论干细胞相关基因Sox-2与宫颈癌的发生相关,其机制可能通过调控Wnt/β-catenin信号通路参与肿瘤的发生发展。

Objective To investigate the expressions of and relationship between Sox-2 and Wnt signaling pathway in cervical squamous carcinoma. Methods The expressions of Sox-2, ^-catenin and C-myc were detected in HeLa, SiHa and C33A, the three cell lines of cervical cancer （CC）, by immunohistochemistry （IHC）. The expressions of these genes were detected in tissues of normal cervix （30 cases） and cervical squamous carcinoma （43 cases） by IHC. Results O Sox-2 was expressed in SiHa and C33A, but not in HeLa; ~3-catenin and C-myc were expressed in HeLa, SiHa and C33A. Q The expressions of Sox-2, ~3-catenin and C-myc were significantly higher in tissues of cervical squamous carcinoma than in tissues of normal cervix （P^0.05）. The expression of Sox-2 in tissues of cervical squamous carcinoma was associated with the histological grade （P = 0. 049）, but not with clinical stage, lymph node metastasis or patient age （P ~ 0. 05）. ~3-catenin and C-myc expressions were not correlated with pathological grade, clinical stage, lymph node metastasis or patient age （P〈0. 05）. In cervical squamous carcinoma, the expression of Sox-2 had a positive correlation with β3-catenin expression （P〈0. 001, r=0. 567） and with C-myc （P=0. 030, r=0. 331）. Conclusion The stem cell marker Sox-2 may be related to the carcinogenesis and progression of cervical carcinoma through the WNT/~3-catenin signaling pathway.