烟草丛顶病伴随RNA临沧分离物基因组全长序列测定和结构分析

Complete Nucleotide Sequence and Genome Organization of A Lincang Isolate of Tobacco Bushy Top Disease-associated RNA

[目的]对烟草丛顶病伴随RNA(TBTDaRNA)临沧分离物基因组全长序列进行测定,并对其进行结构分析。[方法]应用RT-PCR方法扩增烟草丛顶病伴随RNA临沧分离物的基因组,并进行全长序列的测定,同时对烟草丛顶病伴随RNA临沧分离物和龙陵分离物的基因组进行比较分析。[结果]烟草丛顶病伴随RNA临沧分离物和龙陵分离物的基因组全长序列一致性为95.4%;ORF1a编码产物的氨基酸序列一致性为93.1%;ORF1b编码产物的氨基酸序列一致性为98.3%;3'端非编码区的核苷酸序列一致性为95.5%。[结论]该研究为研究TBTDaRNA的分子变异奠定了基础。

[Objeetive] The aim was to sequence the complete nueleotide and analyze the genome organization of a Lineang isolate of Tobacco bushy top disease-associated RNA （TBTDaRNA）. [ Method] The full -length genome of a Lineang isolate of TBTDaRNA was amplified by RT- PCR and sequenced. Comparisons were carried out at the genomes level for TBTDaRNA Lineang isolate and Longling isolate. [ Result] The TBT- DaRNA Lineang isolate and Longling isolate had a 95.4% nueleotide identity in the full - length genomie sequence, and 93.1% and 98.3 % anli- no acid identity between their ORF1 a and ORF1 b, respeetively ; the nueleotide identity between their 3＇untranslated regions was 95.5 %. [ Conclu- sion] The study laid a foundation for the research of molecular variability of TBTDaRNA.