顶空-气质联用法测定竹叶中挥发性成分

Analysis of Volatile Constituents in Bamboo Leaves Using HS-GC /MS

[目的]研究测定竹叶中的挥发性成分。[方法]采用顶空-气质联用方法测定竹叶中的挥发性成分,利用NIST谱库对分离得到的化合物进行结构测定,应用相对峰面积法计算化合物的相对百分含量。[结果]从竹叶中分离得到色谱峰53种,主要以醇类、酮类和醛类化合物为主,且这些化合物中一般含有不饱和键。[结论]应用顶空-气质联用技术能够比较全面、便捷地确定竹叶中的挥发性成分,可为竹叶的进一步开发利用提供科学依据。

[ Objective] This study aimed to investigate the volatile constituents from bamboo leaves. [ Method] The volatile compositions in bamboo leaves were analyzed by headspace gas chromatography mass spectrometry, and identified by their mass spectra using NIST. The rela- tive percentages of them were calculated by the GC peak areas. [ Result] Fifty-three kinds of chemical constituents in bamboo leaves were sep- arated. Among them, the main components were alcohols, ketones and aldehydes compounds, and these compounds contained unsaturated bonds. [ Conclusion] HS-GC/MS was simple and less sample-demanding and can be used for the fast analysis of the volatile constituents of bamboo leaves and provide scientific basis for further research and development of bamboo leaves.