HPLC测定健胃消炎颗粒中大黄素和大黄酚的含量

Determination of Emodin and Chrysophanol in Jianweixiaoyan Particles by HPLC

目的建立健胃消炎颗粒中大黄素和大黄酚含量的测定方法。方法高效液相色谱法。采用Symmetry C18柱(4.6mm×150mm,3.5μm),流动相为甲醇-0.1%磷酸溶液(85∶15)为流动相;检测波长为254nm。结果此方法线性关系良好,无糖型平均加样回收率为99.87%,有糖型平均加样回收率为99.94%。结论此方法简便、准确,可用于测定健胃消炎颗粒中大黄素和大黄酚的含量。

OBJECTIVE To establish an HPLC method for the determination of Emodin and Chrysophanol in Jianweixiaoyan. METHODS The Symmetry Cls column of 4.6mm×150mm,3.5μm was used. The mobile phase consisted of methanol-0. 1% H3 PO4 （ 85：15 ）. The detective wavelength was 254nm. RESULTS The Linearity of this method was well with the sugar-free type average recovery of 99.87% and the sugar tupe average recovery of 99. 94%. CONCLUSION The method is simple and accurate. It can be used for the determination of the contents of Emodin and Chrysophanol in Jianweixiaoyan Particles.