39种动物HMGB1氨基酸序列比对分析

Sequence Alignment of 39 Animal HMGB1 Amino Acid

目的:确定不同动物高迁移率族蛋白B1在物种进化中的关系。方法:运用分子生物学工具软件Generous检索NCBI数据库HMGB1氨基酸序列,比对分析和构建进化树。结果:获得39种动物HMGB1序列,39种动物HMGB1氨基酸序列同源性45.7%,其中哺乳动物序列同源性99.3%。哺乳动物与血吸虫、丝虫、蚊、蜱以及原虫序列同源性分别为:80.2%、80.9%、81.8%、73.3%和53.6%。进化树基本反映了HMGB1与动物物种进化的关系。结论:动物物种间HMGB1具有保守性和高度同源性,在寄生虫与宿主关系中,寄生虫的HMGB1可能是促进宿主炎症反应的一个因素。

Objective：Determining the relationship between animal＇s HMGBls in the evolution of species. Methods：The software Generous,one of molecular biology tool,is used in Searching NCBI database for HMGB1 amino acid,and analyzing the result of sequence alignment, and building phylogenetic tree of animal based on HMGB1. Results.. Found 39 HMGB1 sequence of animals. The percent of Pairwise Identity is 45.7% in 39 sequences of animals HMGB1 amino acid,and which is 99.3% for mammals,80.2% for mammals and schistosomiasis,80.9% for mammals and filariasis,81.8% for mammals and mosquitoes,73.3% for mammals and ticks, 53.6% for mammals and protozoan, respectively. Phylogenetic tree of animal based on HMGB1 reflects the relationship of animal evolution. Conclusion： HMGB1 is highly conserved across species. HMGB1 have a highly homology between the parasite and host. The HMGB1 of parasite may be a factor resulting in host inflammatory response.