补肾益髓胶囊对实验性自身免疫性脑脊髓炎小鼠大脑内BDNF和Nogo A的影响

Effects of Bushen Yisui Capsule on Expression of BDNF and Nogo A in Mice with Experimental Autoimmune Encephalomyelitis

目的观察补肾益髓胶囊对EAE小鼠大脑内脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)和勿动蛋白(Nogo A)表达的影响,探讨本方促进EAE小鼠轴突损伤修复再生的可能机制。方法小鼠背部皮下注射抗原MOG35-55,诱导EAE模型。每天观察小鼠的体质量变化和神经功能评分,发病第18天(发病急性期)和第40天(发病缓解期)取小鼠大脑,HE染色观察其病理改变,用免疫组化(immunohistochemistry,IHC)法测定小鼠大脑中BDNF和Nogo A的变化。结果补肾益髓胶囊明显减轻病灶部位的炎性细胞浸润,明显增加不同时间点BDNF在大脑皮层(P<0.01)、脑室下(P<0.01)及侧脑室旁(P<0.01)的表达,并且可以减少Nogo A在大脑上述不同部位的表达(P<0.05,P<0.01)。结论补肾益髓胶囊的神经保护作用机制可能与其能够促进神经营养因子BDNF及降低抑制因子Nogo A的表达相关。

OBJECTIVE The previous study had shown that Bushen Yisui Capsule played an important role in the develop- ment and prevention of experimental autoimmune encephalomyelitis （EAE）. In present study, the effects of Bushen Yisui Cap- sule on the expression of brain-derived neurotrophic factor （BDNF） and Nogo A in mice with EAE were discussed. METHODS The C57BL/6 mice were induced by immunization with myelin oligodendrocyte glycoprotein （MOG） 35-55. The body weights and neurological function scores of mice were examined everyday. The pathological changes in the brains of mice were observed with hematoxylin-eosin （H^E） staining, while the protein expression of BDNF and Nogo A were measured by im- munohistochemistry （IHC） on Day 18 and Day 40 post-immunization （PI）. RESULTS The data showed that inflammatory cells were reduced in the treatment group. The expression of BDNF obviously increased in the cerebral cortex （P^0.01）, the inferior to third ventricle （P〈0.01） and the around of lateral ventricle （P〈0.01） on Day 18 and Day 40 PI. In addition, the expression of Nogo A obviously decreased in the different areas of brain above （P〈0.05, P〈0.01）. CONCLUSION Bushen Yisui Capsule has neuroprotective function in the mice with EAE, and the mechanism may be related with increasing expression of BDNF and decreasing expression of Nogo A.