归脾汤对抑郁模型大鼠血清中T_3、T_4及TSH含量的影响

The Influence of Guipi Decoction on the Depression Rats Serum Contents of T_3,T_4 and TSH

目的通过测定抑郁模型大鼠血清中T3、T4及TSH含量的变化,探讨归脾汤治疗抑郁症的作用机制。方法实验采用慢性应激方法复制抑郁症大鼠模型。实验动物分为正常组、模型组、百优解组及归脾汤高、中、低剂量组。观察记录大鼠的体质量变化,并根据敞箱实验,对大鼠进行行为学检测。于实验第42天将大鼠处死,股动脉取血,分离血清,利用放射免疫法测定大鼠血清中T3、T4及TSH的含量。结果与正常组比较,模型组大鼠体质量增长速度减慢,水平运动和垂直运动得分减少,血清中T4水平降低,T3及TSH的含量无显著差异。归脾汤各组及百优解组可使模型大鼠体质量增长速度加快,行为学得分增加,提高血清中T4的含量。结论归脾汤能提高模型大鼠血清中T4水平,对慢性应激性刺激大鼠有抗抑郁作用,其机制可能是通过调节HPT轴分泌来实现的。

OBJECTIVE To explore the mechanism of Guipi Decoction treating on the depression rats through the measure- ment of rats serum contents of Triiodothyronine, Thyroxine and Thyrotropin changes. METHODS The depression rat model of depression were established by chronic stress method. Experimental animals were divided into regular group, model group, optimal solution group and Guipi Decoction high, medium and low dose groups. Rats body weight were observed, and open bo- xes in accordance with experiments conducted on rats behavior detection. Till 42 day in the experiment the rats were sacrificed, femoral artery blood, separation of plasma, serum, measured by radioimmunoassay using rat plasma T3, T4 and TSH levels. RESULTS Compared with the regular group, the model group has slower pace of growth of body weight, the scores of hori- zontal movement and vertical movement reduced, blood T4 levels reduced, T3 and TSH levels have remarkable differences. Guipi Decoction in each group and the group optimal solution to increase the growth rate of body weight and movement of be- havior science scores, and can add the blood T4 levels. CONCLUSION Guipi Decoction can add the blood T4 levels on chronic stress-induced irritation rats and as the role of an antidepressant, which may be corrected through the HPT-axis to achieve.