地耳草黄酮类成分UPLC-MS分析

Analytical Study on the Flavonoids Constituents in Herba Hyperici Japonici by UPLC-MS

目的建立超高效液相色谱-质谱仪联用技术(UPLC-MS)对地耳草中黄酮类成分分析的方法。方法采用Alltima C18色谱柱(5μm,250mm×4.6mm,i.d.),以乙腈-0.8%醋酸为流动相,梯度洗脱,流速为0.8mL/min,检测波长为254nm,柱温为30℃。在电喷雾负离子模式下监测采集数据。结果初步鉴定8种黄酮类化合物,并同时测定其中6种黄酮的含量。6种黄酮类化合物的浓度与峰面积的线性关系良好(r2>0.998 2);加样回收率为97.6%～99.7%。结论该方法准确可靠,重现性好,可作为地耳草内在质量评价的依据。

OBJECTIVE To establish an Ultra Performance Liquid Chromatography （UPLC-MS） method to analyze the fla- vonoids constituents in Herba Hyperici Japonici. METHODS The analysis was performed on an Alltima C18 chromatographic column with a gradient solvent system of acetonitrile-0.8% acetic acid at a flow rate of 0.8 mL/min. The detection wavelength was 254 nm and the column temperature was 30 ℃. The data was collected under the negative electrospray ion mode. RESULTS 8 flavonoids constituents being separated and identified, six of them were detected simultaneously. The result shows the de- tected flavonoids constituents have good linearity between concentration and peak area （r2 〉O. 998 2）, and the average recover- ies of the method were between 97.6%～99.7%.CONCLUSION Being simple, accurate and reproducible, UPLC-MS can be used for evaluating the quality of Herba Hyperici Japonici.