骨桥蛋白在胆固醇结石患者胆囊黏膜上皮中的表达及意义

Expression and significance of osteopontin (OPN) protein in gallbladder epithelium of patients with cholesterol gallstone

目的探讨骨桥蛋白(OPN)在胆固醇结石患者胆囊黏膜上皮中的表达、分布及意义。方法采用免疫组化Elivision二步法,检测骨桥蛋白在25例单纯胆囊胆固醇结石患者胆囊黏膜上皮(结石组)和10例非胆囊结石患者的胆囊黏膜上皮(非结石组)中的表达。结果 OPN集中表达于胆囊黏膜上皮细胞的管腔侧,OPN在结石组的表达显著高于非结石组(P<0.05)。结论 OPN在胆固醇结石患者胆囊黏膜上皮内高表达,原因可能与胆固醇结石的形成有关。

Objective To investigate the expression and distribution of osteopontin （OPN） protein in gallbladder epithelium of patients with cholesterol gallstone. Methods The specimens of 25 patients with cholesterol gallstone and 10 controls were studied by immunohistochemical elivision two-step method to detect the expression and distribution of OPN protein. Results OPN was mainly expressed in the luminal side of the gallbladder epithelia cells. The expression of OPN in gallstone group was significantly higher than that in the control group. Conclusion These results suggest that Opn is possibly involved as a core protein in the formation of cholesterol gallstones