液相色谱-质谱法研究附子生物碱的煎煮减毒机理

Exploration of toxicityreducingmechanism of aconite alkaloids duringdecoction process usingliquid chromatography-mass spectrometry

建立了基于液相色谱-质谱分析的附子中生物碱指纹分析方法,并将其应用于附子煎煮减毒的机理研究。基于质谱特征鉴定了生物碱标准品在加热回流过程中产生的6种降解产物,确定了双酯型生物碱的煎煮条件降解规律。在此基础上研究了附子饮片煎煮不同时间条件下生物碱的指纹变化,结果表明,长时间煎煮可明显降低双酯型生物碱和生物脂碱的浓度,为附子的安全用药提供了科学依据。

A high performance liquid chromatography-electrospray ionization mass spectrometry method was developed for the determination of aconite alkaloids.It was used to investigate the degradation of alkaloids of Radix Aconiti Lateralis Preparata during decoction.Six alkaline degradation products were identified,and the degradation regularity of diester-diterpenoid alkaloids was confirmed during the test using standards.The dynamic changes of the amount of aconite alkaloids in the decoction of Radix Aconiti Lateralis Preparata were supervised.Along with the increase of decoction time,the concentrations of diester-diterpenoid alkaloids and lipo-alkaloid decreased significantly.The results can provide a scientific basis for the safety use of aconite. Keywords：