高效液相色谱法同时测定化妆品中的10种合成着色剂

Simultaneous determination of 10 synthetic colorants in cosmetics byhighperformance liquid chromatography

建立了高效液相色谱同时测定化妆品中颜料橙5、酸性黄36、颜料红53、酸性紫49、罗丹明B、溶剂蓝35、苏丹红Ⅱ、苏丹红Ⅳ、分散黄3和颜料红57等10种合成着色剂的方法.用四氢呋喃（THF）、二甲基亚砜（DMSO）和甲醇对样品进行分步超声辅助萃取、离心净化后,在Eclipse XDB-C18（150 mm ×4.6 mm,5μm）色谱柱上分离.用乙腈-0.02 mol/L乙酸铵溶液（用乙酸调pH至4.60）作为流动相进行梯度洗脱,二极管阵列检测器（DAD）检测.在0.5～20.0 mg/L范围内,10种着色剂的峰面积与质量浓度呈线性关系,相关系数（r）大于0.999;定量限（LOQ）为10 ～ 20 mg/kg.在3个添加水平的回收率均在92.9％～ 108.8％之间,相对标准偏差（RSD）为0.5％ ～6.1％（n=6）.该方法简便、快速、灵敏,适合油状、粉状和膏状化妆品中禁限用着色剂的定量检测.

A high performance liquid chromatographic method was developed for the simultaneous determination of 10 synthetic colorants in cosmetics.The cosmetics were extracted by the ultrasonic technique with tetrahydrofuran （THF）,dimethyl sulfoxide （DMSO） and methanol sequentially.Then the extracts were centrifuged for purification and separated on an Eclipse XDB-C18 column （150 mm × 4.6 mm,5 μm） with gradient elution by acetonitrile and 0.02mol/L ammonium acetate （pH 4.60,adjusted with acetic acid）.A diode array detector was used to determine the colorants with the wavelengths ranging from 417 nm to 640 nm.The linear relationships of the 10 colorants between the peak areas and the mass concentrations were obtained in the range of 0.5-20.0 mg/L （r 〉 0.999）.The limits of quantitation ranged from 10 to 20 mg/kg.The average recoveries at three concentration levels ranged from 92.9% to 108.8% with the relative standard deviations in the range of 0.5% to 6.1% （n =6）.The method is simple,rapid and sensitive.It is suitable for the simultaneous determination of the 10 colorants in the oil cosmetics,cream cosmetics and powder cosmetics. Keywords：