高效液相色谱-激光诱导荧光法分析水样中的胺类物质

Analysis of amines in water samples byhighperformance liquid chromatography-laser induced fluorescence detection

通过色谱条件和衍生条件的优化,建立了微量胺类物质的高效液相色谱-激光诱导荧光检测分析方法.该方法灵敏度高,在优化的条件下分析亚精胺、腐胺和组胺,检出限达到10-10 moL/L数量级,且稳定性好.连续进样5次,3种生物胺保留时间的RSD（n=5）小于0.3％,峰面积的RSD（n=5）小于3％,平均加标回收率为94.99％～104.7％.将该方法应用于实际水样中3种生物胺的检测及7种茶叶茶水中胺类物质的分析,取得了良好的结果.该方法灵敏度高,稳定性好,可用于水样中微量胺类物质的分析.

A sensitive high performance liquid chromatography （HPLC）-laser induced fluorescence detection （LIFD） method was developed for the determination of amines.The derivatization and separation conditions were investigated.Under the optimized conditions,spermidine,putrescine and histamine were analyzed.The limits of detection （LODs） of the three biogenic amines （S/N =3） were as low as 10-10 mol/L.This method showed excellent stability.The RSDs of retention times and peak areas of the three biogenic amines were lower than 0.3% and 3%,respectively.This method was applied in biogenic amine analysis in water samples,and the average recoveries were in the range of 94.99%-104.7%.Furthermore,the amines in seven tea samples were analyzed by this method,and satisfactory results were achieved.The developed assay is of excellent sensitivity and good reproducibility,which can be used in the analysis of the amines in water samples.