诃子有效成分组对实验性肝纤维化的影响

Protective effects of effective components group of Medicine Terminalia Fruit on experimental liver fibrosis

目的研究藏药诃子有效成分组(MECG)对四氯化碳(CCl4)诱导的肝纤维化的作用。方法将雄性Wistar大鼠随机分为正常对照组、模型组、秋水仙碱组,以及MECG 90、30和10 mg/kg剂量组。除正常对照组外,其余各组以CCl4与60度白酒1∶2混合物(2 ml/kg,2次/周)灌胃来诱导制备大鼠肝纤维化模型,同时给药组分别给予MECG和阳性药秋水仙碱灌胃,每天1次,持续6周。第6周给药结束后处死大鼠,并解剖观察肝脾外观,对肝、脾称重并计算肝指数及脾指数;采集动脉全血测定大鼠血清天门冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)活性,常规进行肝脏病理学检查。结果与正常对照组相比,给药组和模型组肝脏外观和病理检查符合肝纤维化特征,ALT、AST活性明显升高(P<0.01),肝指数、脾指数也显著增加(P<0.01),提示造模成功;各种剂量给药组与模型组相比,造模后6周大鼠体质量明显增加(P<0.05),MECG 90和30 mg/kg组AST值明显降低(P<0.05),3个剂量组的ALT值均明显好转(P<0.05),且变化呈剂量依赖性;各给药组大鼠肝指数、脾指数异常也较模型组和阳性对照组明显减轻;肝组织病理学检查结果显示,MECG给药组肝细胞变性、坏死及炎性细胞浸润程度均较模型组减轻。结论 EMCG对CCl4诱导的肝纤维化具有保护作用。

Objective To study the protective effect of Medicine Terminalia Fruit effective components group （MECG） on liver fibrosis induced by carbon tetrachloride （ CCl4 ） in rats. Methods Male Wistar rats were randomly divided into normal control group, model group, positive drug （ colchicine ） group, MECG high dose （ 90 mg/kg） , moderate dose （ 30 mg/kg） and low dose （ 10 mg/kg） group. Except for the normal control group, the rest four groups of rats were administrated 1 ： 2 mixture of CCl4 and spirit （2 ml/kg, 2 times per/weeks） to prepare the hepatic fibrosis model. The treatment groups were given different doses of MECG （90 ; 30 and 10 mg/kg） and positive control drug colchicines （0. 2 mg/kg） once a day for six weeks. After six weeks＇ drugs administration, all rats were sacrificed for the collection of blood serum and hepatic tissues. The activities of aspartate transaminase（AST） and alanine transaminase （ALT） in the serum were determined. Besides, the appearances of liver and spleen were observed and their weight were measured, their indexs were counted, and histopathological examinations were performed. Results Compared with normal control group, the appearance and pathological examination results of livers in treatment and model group rats met characteristics of liver fibrosis. The activities of ALT and AST of hepatic fibrosis in rats were significantly higher（ P 〈 0. 01 ） than those in normal control group. The liver index and spleen index had significant difference compared with normal control group （ P 〈 0. 01 ）. It is obvious that this model was successful. Compared with the model group, the body mass of treatedment groups increased significantly （ P 〈 0. 05 ）, the level of AST and ALT in MECG groups was decreased significantly （ P 〈 0. 05 ） , and thir liver index and spleen index were decreased significantly （ P 〈 0. 01 ） in a concentration-dependent manner. The liver pathological histology research showed that the liver cell degeneration, necrosis and degree of inflammatory cells infiltration in MECG group were relieved compared with the model group. Conclusion It is first reported in this paper that MECG can have a better protective effect against CCl4-induced hepatic damage in rats and improve liver function.