透骨消痛方镇痛抗炎作用实验研究

Analgesia and Anti-inflammatory Effects of Tougu Xiaotong Prescription

目的:比较不同方法获得的透骨消痛方各内容物的镇痛抗炎作用,确定最佳处方。方法:采用醋酸扭体法观察透骨消痛方1-4对小鼠镇痛作用的强弱,确定最佳处方,再将最佳处方分为低剂量、中剂量、高剂量3个剂量组,采用醋酸扭体法和二甲苯耳廓肿胀法观察不同药物组对小鼠抗炎镇痛的作用。结果:透骨消痛处方2和处方3均可减少醋酸所致小鼠扭体次数,以处方3为最佳。处方3的中剂量、高剂量组可使小鼠扭体次数显著减少,并对二甲苯所致小鼠耳廓炎症有抑制作用。结论:透骨消痛处方3具有明显的镇痛抗炎作用,可作为新胶囊制备的药物原料。

Objective： To compare the analgesia and anti-inflammatory effects of contents from Tougu Xiaotong Prescription which extracted by different methods and determine the best one.Method： Using the method of acetic acid-induced writhing in mice to observe the effects strength of analgesia from Tougu Xiaotong prescription 1 to 4,the best prescription was determined.Then,the best prescription were divided into low,medium and high dose groups,and the methods of acetic acid-induced writhing and xylene-induced ear swelling in mice were used to observe the anti-inflammation and analgesia effects from three dose groups.Results： Tougu Xiaotong prescription 2 and 3 can reduce the times of acetic acid-induced writhing in mice and the latter was the best.The medium and high dose groups of prescription 3 can significantly reduce the times of acetic acid-induced writhing,and inhibit the xylene-induced ear swelling in mice.Conclusion： The Tougu Xiaotong prescription 3 has a significant anti-inflammation and analgesia effects,which may serve as the raw material for new capsule.