依达拉奉对心室颤动大鼠心肌的保护作用

Myocardial protective effect of edaravone on rats with cardiac arrest

目的 研究依达拉奉对心室颤动（VF）大鼠心肌组织的保护作用.方法 通过食道快速起搏法建立大鼠心室颤动模型,76只SD大鼠随机分为假手术组、对照组、依达拉奉组,每组再分为自主循环恢复（ROSC）后12、24、48及72 h四个时间点.各时间点检测心肌组织超氧化物歧化酶（SOD）、丙二醛（MDA）含量的变化情况,并对心肌行组织病理学光镜检查.结果 依达拉奉组大鼠心肌组织中的SOD含量在12、24、48及72 h均高于对照组（P＜0.01或P＜0.05）,MDA含量在以上四个时间点均低于对照组（P＜0.01或P＜0.05）；镜下观察,依达拉奉组心肌组织病理损伤程度较对照组为轻,其中以24 h和48 h最为明显.结论 ROSC后尽早使用依达拉奉,可通过提升体内SOD活力,降低MDA含量,减轻缺血-再灌注损伤而起到心脏保护作用.

Objective To investigate the myocardial protective role of edaravone in rats with ventricular fibrillation. Methods The rat ventricular fibrillation model was established through rapid oesophageal pacing method. 76 SD rats were randomly divided into sham, control and edaravone group. Each group was subdivided into 12 h, 24 h, 48 h and 72 h four time points after restoration of spontaneous circulation （ROSC）. Superoxide dismutase （SOD） and malondialdehyde （MDA） content in myocardial tissue were detected at each time point. Myocardial histopathological examination was done by light microscopy. Results The edaravone group had a higher content of SOD in myocardial tissue compared with the control group at all four time points （12 h, 24 h, 48 h, 72 h）. But, conversely, MDA content was lower than the control group at the four time points. The pathological damage of myocardial tissue in edaravone group was mild when compared with the control group, which was significant at 24 h and 48 h time points. Conclusion Early use of edaravone after restoration of spontaneous circulation could minimize the revascularization injury of myocardial tissue through elevating the in vivo SOD activity and reducing the MDA level.