新疆维吾尔族和汉族宫颈癌患者微小RNA-143的表达及其临床病理意义

Clinical significance of miR-143 expression in women with cervical cancer of Uyghur and Han ethnicities, in Xinjiang

目的 探讨微小RNA（miR）-143在新疆维吾尔（维）族和汉族宫颈癌患者中的表达及其与临床病理特征的关系.方法 采用qRT-PCR方法检测正常宫颈及宫颈癌组织中miR-143的表达,分析miR-143在维、汉族间的差异及其与临床病理特征的关系.结果 与正常宫颈组比较,miR-143在宫颈癌组中表达显著下降,差异有统计学意义（P＜0.05）,但两民族人群间表达差异无统计学意义（P＞0.05）.miR-143表达与淋巴结转移及肿瘤大小相关.有淋巴结转移的宫颈癌组,miR-143表达明显低于无淋巴结转移的宫颈癌组（Z=-2.127,P=0.033）；肿瘤直径＞4 cm的宫颈癌组miR-143表达明显低于肿块直径≤4 cm的宫颈癌组（Z=-2.628,P=0.009）.ROC曲线下面积（AUC）分析显示,miR-143在不同肿块直径和淋巴结转移的宫颈癌组中,AUC分别为0.711和0.697,均＞0.5.评价癌肿直径的敏感度和特异度分别为85.7％和62.2％；评价淋巴结转移的敏感度和特异度分别为72.2％和60.4％.miR-143表达与患者年龄、癌浸润深度、子宫旁浸润、FIGO分期、组织类型及病理分级无关.结论 miR-143参与宫颈癌的发生发展,且无种族差异表达,可能成为宫颈癌诊断及病情预测的标记物.

Objective To explore the expression of microRNA （miR）-143 and related association to clinicopathologic features of cervical cancer in both Uygur and Han women with cervical cancer in Xinjiang.Methods The expression levels of miR-143 in the specimens from 34 non-tumor and 66 cervical cancer tissues,were examined by quantitative real-time PCR.Levels on the expression of miR-143 in Xinjiang Uygur women and the correlations between the expression levels of miR-143 and related clinicopathologic features of cervical cancer were analyzed.Results The expression levels of miR-143 were significantly lower in the tumor tissues than that in the non-tumor tissues （P〈0.05） but no significant difference was found between women with Uygur or Han ethnicities.Down-regulated miR-143 expression was associated with both the tumor size and lymph node metastasis in patients with cervical cancer （Z=-2.628,P=0.009 and Z=-2.127,P=0.033 respectively）.No significant associations were found between the expression levels of miR-143 and factors as age,depth of tumor invasion,parametrial infiltration,clinical stage,types of histology and stage of differentiation.ROC curve analysis on miR-143 expression in cervical cancer patients with different tumor sizes and lymph node metastasis：the rates on AUC were 0.711 and 0.697,both larger than 0.5,respectively.The sensitivity and specificity of evaluating tumor size were 85.7% and 62.2%.The sensitivity and specificity of lymph node metastasis were 72.2% and 60.4%.Conclusion It seemed that miR-143 play an important role in the processes of generation and progression of cervical cancer.However,there was no significant difference found between the different ethnic groups.The expression level of miR-143 might serve as a valuable adjuvant parameter for diagnosing and predicting the state of cervical cancer.