支气管哮喘患者血清细胞因子水平变化的研究

Research on the changes of serum cytokine levels in patients with bronchial asthma

目的探讨支气管哮喘患者血清细胞因子水平的变化。方法采用酶联免疫吸附法(ELISA)检测支气管哮喘患者在急性发作期、缓解期与正常对照组患者的血清TNF-α、IL-6、IL-8水平及FEV1/FVC值。结果支气管哮喘患者在急性发作期血清TNF-α、IL-6、IL-8水平明显高于缓解期和对照组(P<0.05);缓解期患者的血清TNF-α、IL-6、IL-8显著高于对照组(P<0.05)。支气管哮喘患者在急性发作期、缓解期血清TNF-α、IL-6、IL-8与FEV1/FVC呈负相关(P<0.05)。结论血清TNF-α、IL-6、IL-8水平参与了支气管哮喘的炎症反应,可作为判断支气管哮喘患者病情严重程度的指标。

Objective To explore the changes of serum cytokine levels in patients with bronchial asthma. Methods The enzyme-α inked immunosorbent assay （ELISA） was used to detect the levels of serum TNF-α,IL-6,IL-8 for the patients of the bronchial asthma acute exceration group,asthma remission group and the control group,and detect the data of FEV1/FVC for them. Results The levels of TNF-a,IL-6,IL-8 of bronchial asthma patients in acute execration group were obviously higher than asthma remission group and control group （P〈O.05）：The levels of TNF-cdL-6,IL-8 in asth- ma remission group were significantly higher than control group （P〈0.05）.The levels of TNF-α,IL-6,IL-8 were negatively correlated to the data of FEVI/FVC in bronchial asthma. Conclusion The levels of TNF-α,IL-6,IL-8 are involved in the inflammatory reation of bronchial asthma,can be used as judgement index of patients with bronchial asthma disease severity