出生前后母鼠饮水摄入双酚A对子代未成年期体重的影响

Effect of prenatal and postnatal exposure to bisphenol A on body weight of immature mice

目的探讨母鼠孕期和哺乳期经水摄人双酚A（BPA）对子代未成年期体重的影响。方法将妊娠的ICR母鼠随机分成BPA低、中、高剂量组（2．2829、22．8290、228．2900μg／L）、空白对照组和溶剂对照组；母鼠自妊娠第0天（GD0）至仔鼠出生后第21天（PND 21）经饮水暴露BPA，孕期每3d记录1次母鼠体重，仔鼠体重在哺乳期每3d记录1次，断乳后至PND42期间每周记录1次。结果各BPA暴露组母鼠孕期体重、窝平均仔鼠数及断乳前仔鼠平均体重与对照组相比差异均无统计学意义；PND42时BPA高剂量组仔鼠体重与对照组相比显著增加（P〈0．05）；PND28～PND42时各组雌性仔鼠体重均无明显差异；PND35时中、高剂量组及PND42时BPA各组雄性仔鼠体重与对照组相比均显著增加（P〈0．05，P〈0．01），以PND42时高剂量组增加更为显著。结论母鼠孕期及哺乳期暴露较低剂量BPA对子代未成年期的体重有一定影响，而且对雄性子代影响更明显。

Objective To investigate the effect of maternal exposure to bisphenol A （BPA） via drinking water on body weight of immature offspring during pregnancy and lactation. Methods The pregnant female ICR mice were exposed to BPA groups （2. 282 9,22. 829 0,228. 290 0 μg/L） by drinking water from gestational day （GD）0 till postnatal day （PND）21 ,water and dimethyl sulfoxide（0.01% ） were served as blank and vehicle control. Maternal body weight was recorded every three days during pregnancy. Weight of pups per litter was measured every three days during lactation. Body weight of offspring was taken notes every week till PND 42 after delactation. Results There was no significant difference in pregnant maternal body weight, average numbers and body weight of pups per litter preweaning among all groups. The offspring weight of the high-dose group at PND 42 was increased compared with control groups （ P 〈 0.05 ）. The weight of female offspring was not significantly different among all groups at PND 28 - 42. The male offspring weight of the middle/high group at PND 35 and all groups at PND 42 significantly was elevated （ P 〈 0.05,P 〈 0. 01 ） , and the high group was most significant. Conclusion Body weight of immature offspring is affected, especially male offspring after delectation by pregnant and lactational BPA exposure.