吴茱萸次碱改善溶血性磷脂酰胆碱诱导的内皮细胞缝隙连接细胞间通讯功能障碍

Rutaecarpine prevents the disfunction of gap junction inter-cellular communication induced by LPC in endothelial cells

目的探讨吴茱萸次碱(rutaecarpine,Rut)抗溶血性磷脂酰胆碱(LPC)损伤的内皮保护效应及对缝隙连接细胞间通讯功能(GJIC)的调节作用。方法在培养的人脐静脉内皮细胞(HUVEC),预先加入不同浓度的Rut(1.0、0.3、0.1μmol·L-1),处理10 min后加入LPC(10 mg·L-1)共同孵育24 h。应用辣椒素受体(TRPV1)竞争性拮抗剂CAPZ(10μmol·L-1)研究TRPV1是否介导Rut的保护效应。荧光定量PCR检测缝隙连接蛋白Cx37、Cx40的mRNA水平,Western blot检测Cx37和Cx40的蛋白水平,划痕负载试验测定GJIC功能。检测内皮细胞活力(MTT法)、活性氧(ROS)水平和细胞培养液中NO水平及单核细胞黏附,以评价内皮细胞损伤程度。结果 LPC明显下调HUVEC中Cx37和Cx40的mRNA和蛋白水平,抑制缝隙连接染料迁移。而Rut可明显恢复Cx37、Cx40的表达,改善GJIC功能,并减轻LPC诱导血管内皮损伤,表现为增加细胞活力和NO水平,抑制ROS生成和单核细胞黏附。预先给予CAPZ可取消这些效应。结论 Rut可抑制LPC诱导的内皮细胞损伤,恢复Cx37和Cx40的表达而改善GJIC,其机制与激活TRPV1有关。

Aim To investigate the protective effect of rutaecarpine against endothelial cell damage and the dysfuction of gap junction induced by lysopbosphatidylcholine（LPC）. Methods Cell damage was induced by treatment with LPC （ 10 mg · L^-1 ） for 24 h. HUVECs were pretreated with rutaecarpine （1.0,0.3,0. 1μmol·L^-1 ） 10 min before LPC treatment. To explore whether the protective effects of rutaecarpine on HUVECs involved TRPV1, the TRPVl competitive antagonist CAPZ （ 10 μmol·L^-1） was used. The level of Cx37 and Cx40 mRNA was detected by Real-Time PCR, protein level was measured by Western blot, and the function of gap junction inter-cellular communication（GJIC） was determined by intercellular transfer of Lucifer yellow. The endothelial cell injury was evaluated by cell viability （MTT）, production of ROS, the level of NO in the medium, and the adhesion ratio of monocyte-endothelial-cell. Results Exposure of HU- VECs to LPC significantly decreased the expression of Cx37 and Cx40, both the level of mRNA and protein, and inhibited intercellular transfer of Lucifer yellow. Pretreatment with rutaecarpine significantly upregulated the expression of Cx37 and Cx40, and improved the function of GJIC. Rutaeearpine attenuated the endothelial cell injury, which included increasing the cell viability and NO production, decreasing the production of ROS, and inhibiting the monocyte adhesion. Pretreatment with CAPZ abolished the protective effect of rutaecarpine on gap junction and endothelial cells. Conclusion Rutaecarpine prevents the endothelial cell injury induced by LPC, and improves the function of GJIC by increasing the expression of Cx37 and Cx40. These effects are related to the activation of TRPV1.