抗生素浓度对不同品种马铃薯脱毒苗农艺性状的影响

Effect of antibiotic concentration on agronomic traits of different varieties virus-free potato plantlets

以马铃薯不同基因型品种脱毒基础苗为试验材料,采用两因素随机区组设计,研究不同基因型品种、不同抗生素浓度对马铃薯脱毒基础苗苗鲜重、根鲜重、苗干重、根干重、叶片数、节数、株高、茎粗、根长和根条数等农艺性状影响,培养14 d时,测定各性状指标。结果表明:3个不同成熟期基因型品种对测定的10个农艺性状指标影响显著(P<0.05);4种抗生素浓度(0、20、40、60 mg·L-1)除了茎粗,对其它9个农艺性状指标影响显著(P<0.05),适宜的抗生素浓度范围为0 mg·L-1和20 mg·L-1;3个不同成熟期基因型品种、4种不同抗生素浓度处理对马铃薯单株苗鲜重、根鲜重、苗干重、根干重、节数、株高、茎粗、根条数影响有显著性交互作用(P<0.05),叶片数、根长不受基因型品种和抗生素浓度交互作用影响;早熟基因型品种大西洋、中熟基因型品种克新1和晚熟基因型品种青薯168适宜的抗生素浓度为0、20 mg·L-1,各农艺性状表现好。为预防污染发生,降低生产成本,建议继代3-5代时,加入抗生素20 mg·L-1。

The different varieties of virus-free potato plantlets were selected as the meterials ,and using the method of randomized block design of two factor to study the effects of genotypes and different antibiotic concentrations on the fresh weight of shoot and root ,the dry weight of shoot and root ,leaf number ,node number ,plant height ,stem diame-ter ,root length and root number of individuals after 14 d culture .Our results showed that genotypes had significant ef-fects on the ten agronomic traits （ P〈0 .05 ） .Four kinds of antibiotic concentrations （0、20、40、60 mg·L-1 ）had notable interaction effects on other agronomic characters eccept stem diameter （ P〈0 .05 ） ,the suitable antibiotic concentrations were 0 mg·L-1 and 20 mg·L-1 .Except for leaf number and root length properties index ,the genotypes and antibiotic concentrationshad notable interactions on other agronomic characters eccept （ P〈0 .05 ） .The suitable antibiotic concen-trations for the early-maturing varietie“Atlantic” ,medium-maturing varietie“Kexin 1”and late-maturing varietie“Qing-shu 168”were 0 mg·L-1 and 20 mg·L-1 ,to prevent the pollution and production reduction ,we suggested to add 20 mg ·L-1 antibiotic if subcultured for 3-5 generations .