引发对黄芪种子萌发及幼苗抗旱性的影响

Effects of different priming treatments on germination and seedling drought-resistances of Astragalus membranaceus(Fisch)Bge.

本研究目的在于分析种子引发处理后黄芪发芽特性及幼苗抗旱性,探索提高黄芪种子萌发率的方法,为人工栽培黄芪提供技术支撑。采用20%PEG-6000、0.25%KNO3、100 mg·L-1GA3、12.5 mg·L-16-BA及H2O(对照)5种化学物质引发处理黄芪种子,进行萌发试验及干旱胁迫,测定种子萌发特性及有关抗旱生理指标。结果表明:各处理发芽势、发芽率的一致顺序为12.5 mg·L-16-BA>100 mg·L-1GA3>0.25%KNO3>20%PEG-6000>H2O。幼苗丙二醛(MDA)含量、可溶性蛋白含量、脯氨酸含量、叶绿素含量、超氧化物歧化酶活性(SOD)、过氧化物酶活性(POD)和过氧化氢酶活性(CAT)与CK之间存在显著差异。比较各处理黄芪幼苗的隶属函数平均值可看出,不同引发处理黄芪种子,幼苗的抗旱性顺序为H2O>20%PEG-6000>0.25%KNO3U12.5 mg·L-16-BA>CK>100 mg·L-1GA3。结论:采用20%PEG-6000或0.25%KNO3作为引发剂处理黄芪种子,可有效促进黄芪种子萌发和提高幼苗抗旱性。

Objective：Physiological effects of seed priming on drought resistance of Astragalus membranaceus （Fisch .） Bge .seedlings were studied in this paper .Method：After Astragalus membranaceus （Fisch .） Bge .seeds were subject to different priming with H2O ,20% PEG-6000 ,0 .25% KNO3 ,100 mg·L-1 GA3 and 12 .5 mg·L-1 6-BA respectively for germination experiment , seedlings were under 20% PEG -6000 simulated drought stress to measure drought-related physiological indicators .Results：The sequence of Germination energy and germination percentage was i-dentical ：12 .5 mg·L-1 6-BA〉100 mg·L-1 GA3〉0 .25% KNO3〉20% PEG-6000〉H2O〉CK;and the sequence of Simplified vigor index was 0 .25% KNO3〉100 mg·L-1 GA3≈20% PEG-6000〉H2O〉12 .5 mg·L-1 6-BA〉CK .Malondialdehyde （MDA ） content ,soluble protein content ,proline content ,chlorophyll content ,superoxide dismu-tase （SOD ） ,peroxidase （POD ） and catalase activity （CAT ） of seedling have a significant difference between CK .Ac-cording to comparing the Average value of subordinate function ,the rank of drought resistance was H2O〉20% PEG-6000〉0 .25% KNO3≈12 .5 mg·L-1 6-BA〉CK〉100 mg·L-1 GA3 .Conclusion ：20% PEG -6000 ,or 0 .25%KNO3 can effectively promote seed germination and improve seedling drought-resistance .