摘要
本研究目的在于分析种子引发处理后黄芪发芽特性及幼苗抗旱性,探索提高黄芪种子萌发率的方法,为人工栽培黄芪提供技术支撑。采用20%PEG-6000、0.25%KNO3、100 mg·L-1GA3、12.5 mg·L-16-BA及H2O(对照)5种化学物质引发处理黄芪种子,进行萌发试验及干旱胁迫,测定种子萌发特性及有关抗旱生理指标。结果表明:各处理发芽势、发芽率的一致顺序为12.5 mg·L-16-BA>100 mg·L-1GA3>0.25%KNO3>20%PEG-6000>H2O。幼苗丙二醛(MDA)含量、可溶性蛋白含量、脯氨酸含量、叶绿素含量、超氧化物歧化酶活性(SOD)、过氧化物酶活性(POD)和过氧化氢酶活性(CAT)与CK之间存在显著差异。比较各处理黄芪幼苗的隶属函数平均值可看出,不同引发处理黄芪种子,幼苗的抗旱性顺序为H2O>20%PEG-6000>0.25%KNO3U12.5 mg·L-16-BA>CK>100 mg·L-1GA3。结论:采用20%PEG-6000或0.25%KNO3作为引发剂处理黄芪种子,可有效促进黄芪种子萌发和提高幼苗抗旱性。
Objective:Physiological effects of seed priming on drought resistance of Astragalus membranaceus (Fisch .) Bge .seedlings were studied in this paper .Method:After Astragalus membranaceus (Fisch .) Bge .seeds were subject to different priming with H2O ,20% PEG-6000 ,0 .25% KNO3 ,100 mg·L-1 GA3 and 12 .5 mg·L-1 6-BA respectively for germination experiment , seedlings were under 20% PEG -6000 simulated drought stress to measure drought-related physiological indicators .Results:The sequence of Germination energy and germination percentage was i-dentical :12 .5 mg·L-1 6-BA〉100 mg·L-1 GA3〉0 .25% KNO3〉20% PEG-6000〉H2O〉CK;and the sequence of Simplified vigor index was 0 .25% KNO3〉100 mg·L-1 GA3≈20% PEG-6000〉H2O〉12 .5 mg·L-1 6-BA〉CK .Malondialdehyde (MDA ) content ,soluble protein content ,proline content ,chlorophyll content ,superoxide dismu-tase (SOD ) ,peroxidase (POD ) and catalase activity (CAT ) of seedling have a significant difference between CK .Ac-cording to comparing the Average value of subordinate function ,the rank of drought resistance was H2O〉20% PEG-6000〉0 .25% KNO3≈12 .5 mg·L-1 6-BA〉CK〉100 mg·L-1 GA3 .Conclusion :20% PEG -6000 ,or 0 .25%KNO3 can effectively promote seed germination and improve seedling drought-resistance .
出处
《干旱地区农业研究》
CSCD
北大核心
2013年第5期233-237,共5页
Agricultural Research in the Arid Areas
基金
"十二五"国家科技支撑计划(2011BAD29B04)
陕西省科技统筹创新工程(2011KTCL02-02)
关键词
黄芪
引发处理
萌发
抗旱性
Astragalus membranaceus (Fisch.) Bge. seed
Priming treatments
Permination
Drought-resistance