超声萃取-反相高效液相色谱法测定不同产地青蒿中青蒿素的含量

Ultrasonic Extraction RP-HPLC Determination of Artemisinin in Artemisia Annua L.from Different Origins

采用反相高效液相色谱法测定青蒿中青蒿素,并评价了不同产地青蒿中青蒿素含量的差异。样品经石油醚超声提取10 min,采用Kromasil C_(18)色谱柱(150 mm×4.6 mm,5μm)分离,以62比38的体积比混合的甲醇和pH 5.8乙酸盐缓冲溶液为流动相洗脱,检测波长为260 nm。测得青蒿素的质量浓度在10~40 mg·L^(-1)范围内与峰面积呈线性关系,检出限(3s/k)为4.03×10^(-3)mg·L^(-1)。经比较分析试验结果发现,不同省份的青蒿素含量差异很大,湖南怀化产青蒿中青蒿素含量最高(w=0.222%);同一省份不同地区产青蒿中青蒿素的含量也有差异,本地家种的青蒿中青蒿素含量大于本地野生的。

RP HPLC was used to determine the content of artemisinin in Artemisia annua L. and to evalueate the difference of contents of artemisinin from different origins. The sample was ultrasonically extracted with petroleum ether for 10 rain, and separated on Kromasil C18 （150 mm×4.6 mm, 5μm） column, using a mixture of methanol and acetate buffer solution of pH 5. 8 mixed in the ratio of 62 to 38 as mobile phase. UV detection was made at the wavelength of 260 nm. Linear relationship between values of peak area and mass concentration was obtained in the range of 10-40 mg· L^-1, with detection limit （3s/k） of 4.03×10 ^3mg· L^-1. It was shown that：① great differences of contents of artemisinin were found among samples of Artemisia annua L. from various provinces, with the highest content of 0. 222% in sample from the city of Huaihua in Hunan province; ② contents of artemisinin of samples from various regions in a same province were also found to be different and ③ as compared with the wild-grown Artemisia annua L. samples, the content of artemisinin in artificially cultivated samples was much higher.