人脑胶质瘤组织中血管内皮生长因子表达及血管生成拟态的观察

Expression of VEGF and existense of vasculogenic mimicry in human glioma tissues

目的探讨人脑胶质瘤组织中血管内皮生长因子(vascular endothelial growth factor,VGEF)的表达及意义;同时观测胶质瘤组织中是否存在血管生成拟态及其临床意义。方法应用免疫组织化学S-P法检测58例胶质瘤组织中VGEF蛋白阳性表达情况并进行统计学分析;对瘤体组织行CD34-PAS双染色,观察肿瘤血管形态。结果 58例不同级别的胶质瘤组织中,VGEF蛋白阳性表达率分别为2/6、9/19、14/21和9/12。Ⅰ、Ⅱ级与Ⅲ、Ⅳ级组织比较,差异有统计学意义(χ2=3.87,P<0.05);在部分双重染色的组织切片上观察到典型的血管生成拟态图像。结论在胶质瘤组织中存在VGEF高表达现象及血管生成拟态现象,并与胶质瘤分化程度有关。

Objective To investigate the expressions of VEGF in glioma tissues and whether vasculogenic mimicry（VM） exists in glioma and explain the clinical significance of VM. Methods 58 patients with glioma tissues were studied by using S-P method,and the expressions of VEGF were detected; by using CD34-PAS dual staining,the positive rates were compared and observed. Results The positive rates of VEGF expressions in gliomas of gradesⅠ,Ⅱ ,Ⅲ, Ⅳ were 1/6,9/19,14/21 and 12/9,there was significant difference between gradeⅠ,Ⅱ and grade Ⅲ, Ⅳ （χ2=3.87,P〈0.05）; the typical patterns of vasculogenic mimicry were observed. Conclusions There is high expression of VEGF in glioma related to glioma differention; vasculogenic mimicry exists in glioma,and the higher invasive glioma the more VM.