基于聚邻苯二胺复合膜电位型免疫传感器的制备

Preparation of Potentiornetric Immunosensor Based on Poly (o-Phenylenediamine) Film

首先利用循环伏安法制备聚邻苯二胺(POPD)修饰碳糊电极(CPE/POPD),然后在CPE/POPD上电沉积普鲁士蓝(PB)和纳米金(GNPs),制备CPE/POPD/PB-GNPs电极,最后将羊抗小鼠IgG通过金-氨键固定在CPE/POPD/PB-GNPs上,从而制得一种免疫传感器(CPE/POPD/PB-GNPs/Ab).用循环伏安法和电化学交流阻抗技术对电极的修饰过程进行表征.利用所制备的免疫传感器对溶液中的小鼠IgG进行检测,结果表明,在2.0×10-5μg/L和1.0×04μg/L 2种浓度下,免疫传感器均得到了很稳定的电位响应信号,响应时间不超过3min.所制备的免疫传感器具有灵敏度高、响应速度快、检测范围宽、成本低廉等突出优点.

Firstly,o-phenylenediamine（OPD） was electropolymerized on carbon paste electrode（CPE / POPD） by cyclic voltammetry method.Then the gold nanoparticals（GNPs） and prussian blue（PB） were deposited onto the CPE / POPD by electrodeposition technology to fabricate CPE / POPD / PB-GNPs electrode.Finally,the goat anti-mouse IgG（antibody,Ab） were immobilizeded on to the CPE / POPD / PBGNPs electrode through Au-NH-bond,and thus a potentiometric immunosensor（CPE / POPD / PB-GNPs / Ab） was sucessfully prepared.The modified process of the electrode was characterized by cyclic voltammetry（CV） and electrochemical impedance spectroscopy（EIS）.The immunosensor exhibited fast potentiometric response（〈3 min） under the antigen concentration of 2.0×10-5 μ g / L and 1.0×10 4 μ g / L when it was used to detect the mouse IgG.The results of experiments showed that the immunosensor based on POPD / GNPs-PB composite film exhibited the advantages of high sensitivity,fast response,wide detection range and low cost.