小鼠卵巢颗粒细胞条件培养液中蛋白质和激素水平

Induce capacitation of two different mouse granulose cell conditional media

测定用于精子顶体反应和早期胚胎离体培养的小鼠卵巢颗粒细胞条件培养液中的蛋白质和激素水平。结果表明 :单层培养的颗粒细胞体外培养后生长良好 ,能稳定分泌蛋白质、雌二醇( E2 )和孕酮 ( P)。在培养液中加入促卵泡素 ( FSH)和胰岛素 ( INS)时 ,E2的分泌量无显著变化。

Two different conditional culture media of mouse granulosa cells were used either for stimulating sperm aerosome reactor or for early embryos cultured in vitro.The resluts showed that the developing condition of granulosa cells in vitro in our laborotry was the same as in other reports.The induce of Protein,Estradiol 17b biosynthesis and Progesterone was stable , the capacity of Estradiol 17b biosynthesis made no significant difference.